气候变化领域集成服务门户(CCPortal): A constitutively monomeric UVR8 photoreceptor confers enhanced UV-B photomorphogenesis

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DOI	10.1073/pnas.2017284118
	A constitutively monomeric UVR8 photoreceptor confers enhanced UV-B photomorphogenesis
	Podolec R.; Lau K.; Wagnon T.B.; Hothorn M.; Ulm R.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	The plant ultraviolet-B (UV-B) photoreceptor UVR8 plays an important role in UV-B acclimation and survival. UV-B absorption by homodimeric UVR8 induces its monomerization and interaction with the E3 ubiquitin ligase COP1, leading ultimately to gene expression changes. UVR8 is inactivated through redimerization, facilitated by RUP1 and RUP2. Here, we describe a semidominant, hyperactive allele, namely uvr8-17D, that harbors a glycine-101 to serine mutation. UVR8G101S overexpression led to weak constitutive photomorphogenesis and extreme UV-B responsiveness. UVR8G101S was observed to be predominantly monomeric in vivo and, once activated by UV-B, was not efficiently inactivated. Analysis of a UVR8 crystal structure containing the G101S mutation revealed the distortion of a loop region normally involved in stabilization of the UVR8 homodimer. Plants expressing a UVR8 variant combining G101S with the previously described W285A mutation exhibited robust constitutive photomorphogenesis. This work provides further insight into UVR8 activation and inactivation mechanisms and describes a genetic tool for the manipulation of photomorphogenic responses. © This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Abiotic stress; Acclimation; Photoreceptor; Signal transduction; UV-B
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180750
作者单位	Department of Botany and Plant Biology, Section of Biology, Faculty of Sciences, University of Geneva, Geneva 4, CH-1211, Switzerland; Institute of Genetics, Genomics of Geneva, University of Geneva, Geneva 4, CH-1211, Switzerland; School of Life Sciences, Protein Production and Structure Core Facility, École Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland
推荐引用方式 GB/T 7714	Podolec R.,Lau K.,Wagnon T.B.,et al. A constitutively monomeric UVR8 photoreceptor confers enhanced UV-B photomorphogenesis[J],2021,118(6).
APA	Podolec R.,Lau K.,Wagnon T.B.,Hothorn M.,&Ulm R..(2021).A constitutively monomeric UVR8 photoreceptor confers enhanced UV-B photomorphogenesis.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Podolec R.,et al."A constitutively monomeric UVR8 photoreceptor confers enhanced UV-B photomorphogenesis".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).