气候变化领域集成服务门户(CCPortal): In vivo NIR-II structured-illumination light-sheet microscopy

CCPortal

DOI	10.1073/pnas.2023888118
	In vivo NIR-II structured-illumination light-sheet microscopy
	Wang F.; Ma Z.; Zhong Y.; Salazar F.; Xu C.; Ren F.; Qu L.; Wu A.M.; Dai H.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Noninvasive optical imaging with deep tissue penetration depth and high spatiotemporal resolution is important to longitudinally studying the biology at the single-cell level in live mammals, but has been challenging due to light scattering. Here, we developed near-infrared II (NIR-II) (1,000 to 1,700 nm) structured-illumination light-sheet microscopy (NIR-II SIM) with ultralong excitation and emission wavelengths up to ∼1,540 and ∼1,700 nm, respectively, suppressing light scattering to afford large volumetric three-dimensional (3D) imaging of tissues with deep-axial penetration depths. Integrating structured illumination into NIR-II light-sheet microscopy further diminished background and improved spatial resolution by approximately twofold. In vivo oblique NIR-II SIM was performed noninvasively for 3D volumetric multiplexed molecular imaging of the CT26 tumor microenvironment in mice, longitudinally mapping out CD4, CD8, and OX40 at the single-cell level in response to immunotherapy by cytosine-phosphate-guanine (CpG), a Toll-like receptor 9 (TLR-9) agonist combined with OX40 antibody treatment. NIR-II SIM affords an additional tool for noninvasive volumetric molecular imaging of immune cells in live mammals. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Light-sheet microscope; Near-infrared II imaging; Noninvasive imaging; Structured-illumination microscopy
语种	英语
scopus关键词	CD134 antigen; cytosine; guanine; phosphate; toll like receptor 9; animal experiment; animal model; animal tissue; Article; cancer immunotherapy; CD4+ T lymphocyte; CD8+ T lymphocyte; controlled study; fluorescence imaging; in vivo study; light scattering; microscopy; mouse; near infrared spectroscopy; nonhuman; priority journal; tumor microenvironment; volumetry
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180739
作者单位	Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Bio-X, Stanford University, Stanford, CA 94305, United States; Molecular Imaging and Therapy, Beckman Research Institute, City of Hope, Duarte, CA 91010, United States; School of Medicine, Stanford University, Stanford, CA 94303, United States
推荐引用方式 GB/T 7714	Wang F.,Ma Z.,Zhong Y.,et al. In vivo NIR-II structured-illumination light-sheet microscopy[J],2021,118(6).
APA	Wang F..,Ma Z..,Zhong Y..,Salazar F..,Xu C..,...&Dai H..(2021).In vivo NIR-II structured-illumination light-sheet microscopy.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Wang F.,et al."In vivo NIR-II structured-illumination light-sheet microscopy".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).