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DOI	10.1073/pnas.2016903118
	Redox regulation of NADP-malate dehydrogenase is vital for land plants under fluctuating light environment
	Yokochi Y.; Yoshida K.; Hahn F.; Miyagi A.; Wakabayashi K.-I.; Kawai-Yamada M.; Weber A.P.M.; Hisabori T.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Many enzymes involved in photosynthesis possess highly conserved cysteine residues that serve as redox switches in chloroplasts. These redox switches function to activate or deactivate enzymes during light-dark transitions and have the function of fine-tuning their activities according to the intensity of light. Accordingly, many studies on chloroplast redox regulation have been conducted under the hypothesis that “fine regulation of the activities of these enzymes is crucial for efficient photosynthesis.” However, the impact of the regulatory system on plant metabolism is still unclear. To test this hypothesis, we here studied the impact of the ablation of a redox switch in chloroplast NADP-malate dehydrogenase (MDH). By genome editing, we generated a mutant plant whose MDH lacks one of its redox switches and is active even in dark conditions. Although NADPH consumption by MDH in the dark is expected to be harmful to plant growth, the mutant line did not show any phenotypic differences under standard long-day conditions. In contrast, the mutant line showed severe growth retardation under short-day or fluctuating light conditions. These results indicate that thiol-switch redox regulation of MDH activity is crucial for maintaining NADPH homeostasis in chloroplasts under these conditions. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Chloroplast; Genome editing; NADP-malate dehydrogenase; Redox regulation; Thioredoxin
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180735
作者单位	Laboratory of Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, 226-8503, Japan; School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, 226-8503, Japan; Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences, Center for Synthetic Life Sciences, Heinrich Heine University Düsseldorf, Düsseldorf, 40225, Germany; Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Saitama, 338-8570, Japan; Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom
推荐引用方式 GB/T 7714	Yokochi Y.,Yoshida K.,Hahn F.,et al. Redox regulation of NADP-malate dehydrogenase is vital for land plants under fluctuating light environment[J],2021,118(6).
APA	Yokochi Y..,Yoshida K..,Hahn F..,Miyagi A..,Wakabayashi K.-I..,...&Hisabori T..(2021).Redox regulation of NADP-malate dehydrogenase is vital for land plants under fluctuating light environment.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Yokochi Y.,et al."Redox regulation of NADP-malate dehydrogenase is vital for land plants under fluctuating light environment".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).