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DOI	10.1073/pnas.2005256118
	The arabidopsis GRAS-type SCL28 transcription factor controls the mitotic cell cycle and division plane orientation
	Goldy C.; Pedroza-Garcia J.-A.; Breakfield N.; Cools T.; Vena R.; Benfey P.N.; de Veylder L.; Palatnik J.; Rodriguez R.E.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Gene expression is reconfigured rapidly during the cell cycle to execute the cellular functions specific to each phase. Studies conducted with synchronized plant cell suspension cultures have identified hundreds of genes with periodic expression patterns across the phases of the cell cycle, but these results may differ from expression occurring in the context of intact organs. Here, we describe the use of fluorescence-activated cell sorting to analyze the gene expression profile of G2/M cells in the growing root. To this end, we isolated cells expressing the early mitosis cell cycle marker CYCLINB1;1-GFP from Arabidopsis root tips. Transcriptome analysis of these cells allowed identification of hundreds of genes whose expression is reduced or enriched in G2/M cells, including many not previously reported from cell suspension cultures. From this dataset, we identified SCL28, a transcription factor belonging to the GRAS family, whose messenger RNA accumulates to the highest levels in G2/M and is regulated by MYB3R transcription factors. Functional analysis indicates that SCL28 promotes progression through G2/M and modulates the selection of cell division planes. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Arabidopsis; Cell cycle; Root meristem
语种	英语
scopus关键词	messenger RNA; protein SCL28; transcription factor; transcriptome; unclassified drug; Arabidopsis; Article; cell cycle G2 phase; cell cycle M phase; cell division; cell suspension; confocal microscopy; controlled study; fluorescence activated cell sorting; gene expression; mitosis; nonhuman; phenotype; plant root; priority journal; protein analysis; reverse transcription polymerase chain reaction
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180734
作者单位	Instituto de Biología Molecular y Celular de Rosario, National Scientific and Technical Research Council, Universidad Nacional de Rosario, Rosario, 2000, Argentina; Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, 9052, Belgium; Center for Plant Systems Biology, Vlaams Instituut Voor Biotechnologie, Gent, 9052, Belgium; HHMI, Duke University, Durham, NC 27708, United States; Department of Biology, Duke University, Durham, NC 27708, United States; Centro de Estudios Interdisciplinarios, Universidad Nacional de Rosario, Rosario, 2000, Argentina
推荐引用方式 GB/T 7714	Goldy C.,Pedroza-Garcia J.-A.,Breakfield N.,et al. The arabidopsis GRAS-type SCL28 transcription factor controls the mitotic cell cycle and division plane orientation[J],2021,118(6).
APA	Goldy C..,Pedroza-Garcia J.-A..,Breakfield N..,Cools T..,Vena R..,...&Rodriguez R.E..(2021).The arabidopsis GRAS-type SCL28 transcription factor controls the mitotic cell cycle and division plane orientation.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Goldy C.,et al."The arabidopsis GRAS-type SCL28 transcription factor controls the mitotic cell cycle and division plane orientation".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).