气候变化领域集成服务门户(CCPortal): A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed

CCPortal

DOI	10.1073/pnas.2020235118
	A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed
	Alich T.C.; Pabst M.; Pothmann L.; Szalontai B.; Faas G.C.; Mody I.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Voltage sensing with genetically expressed optical probes is highly desirable for large-scale recordings of neuronal activity and detection of localized voltage signals in single neurons. Most genetically encodable voltage indicators (GEVI) have drawbacks including slow response, low fluorescence, or excessive bleaching. Here we present a dark quencher GEVI approach (dqGEVI) using a Förster resonance energy transfer pair between a fluorophore glycosylphosphatidylinositol-enhanced green fluorescent protein (GPI-eGFP) on the outer surface of the neuronal membrane and an azo-benzene dye quencher (D3) that rapidly moves in the membrane driven by voltage. In contrast to previous probes, the sensor has a single photon bleaching time constant of ∼40 min, has a high temporal resolution and fidelity for detecting action potential firing at 100 Hz, resolves membrane de- and hyperpolarizations of a few millivolts, and has negligible effects on passive membrane properties or synaptic events. The dqGEVI approach should be a valuable tool for optical recordings of subcellular or population membrane potential changes in nerve cells. © This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Cultured neurons; Fluorescent membrane potential measurement; Genetically encoded voltage indicator; GEVI
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180731
作者单位	Neuronal Networks in Health and Disease Laboratory, Institute of Experimental Epileptology and Cognition Research, Life and Brain Center, University of Bonn Medical Center, Bonn, 53127, Germany; Department of Neurology, The David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, United States
推荐引用方式 GB/T 7714	Alich T.C.,Pabst M.,Pothmann L.,et al. A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed[J],2021,118(6).
APA	Alich T.C.,Pabst M.,Pothmann L.,Szalontai B.,Faas G.C.,&Mody I..(2021).A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Alich T.C.,et al."A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).