气候变化领域集成服务门户(CCPortal): Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization

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DOI	10.1073/pnas.2017709118
	Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization
	Foley M.H.; O'Flaherty S.; Allen G.; Rivera A.J.; Stewart A.K.; Barrangou R.; Theriot C.M.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Primary bile acids (BAs) are a collection of host-synthesized metabolites that shape physiology and metabolism. BAs transit the gastrointestinal tract and are subjected to a variety of chemical transformations encoded by indigenous bacteria. The resulting microbiota-derived BA pool is a mediator of host-microbiota interactions. Bacterial bile salt hydrolases (BSHs) cleave the conjugated glycine or taurine from BAs, an essential upstream step for the production of deconjugated and secondary BAs. Probiotic lactobacilli harbor a considerable number and diversity of BSHs; however, their contribution to Lactobacillus fitness and colonization remains poorly understood. Here, we define and compare the functions of multiple BSHs encoded by Lactobacillus acidophilus and Lactobacillus gasseri. Our genetic and biochemical characterization of lactobacilli BSHs lend to a model of Lactobacillus adaptation to the gut. These findings deviate from previous notions that BSHs generally promote colonization and detoxify bile. Rather, we show that BSH enzymatic preferences and the intrinsic chemical features of various BAs determine the toxicity of these molecules during Lactobacillus growth. BSHs were able to alter the Lactobacillus transcriptome in a BA-dependent manner. Finally, BSHs were able to dictate differences in bacterial competition in vitro and in vivo, defining their impact on BSH-encoding bacteria within the greater gastrointestinal tract ecosystem. This work emphasizes the importance of considering the enzymatic preferences of BSHs alongside the conjugated/deconjugated BA-bacterial interaction. These results deepen our understanding of the BA-microbiome axis and provide a framework to engineer lactobacilli with improved bile resistance and use probiotics as BA-altering therapeutics. © This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Acidophilus; Bile acid; Bile salt hydrolase; Gasseri; Lactobacillus
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180728
作者单位	Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27695, United States; Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, United States; Molecular Education Technology and Research Innovation Center, North Carolina State University, Raleigh, NC 27695, United States
推荐引用方式 GB/T 7714	Foley M.H.,O'Flaherty S.,Allen G.,et al. Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization[J],2021,118(6).
APA	Foley M.H..,O'Flaherty S..,Allen G..,Rivera A.J..,Stewart A.K..,...&Theriot C.M..(2021).Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Foley M.H.,et al."Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).