气候变化领域集成服务门户(CCPortal): Conjugative plasmids interact with insertion sequences to shape the horizontal transfer of antimicrobial resistance genes

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DOI	10.1073/pnas.2008731118
	Conjugative plasmids interact with insertion sequences to shape the horizontal transfer of antimicrobial resistance genes
	Che Y.; Yang Y.; Xu X.; Brinda K.; Polz M.F.; Hanage W.P.; Zhang T.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	It is well established that plasmids play an important role in the dissemination of antimicrobial resistance (AMR) genes; however, little is known about the role of the underlying interactions between different plasmid categories and other mobile genetic elements (MGEs) in shaping the promiscuous spread of AMR genes. Here, we developed a tool designed for plasmid classification, AMR gene annotation, and plasmid visualization and found that most plasmid-borne AMR genes, including those localized on class 1 integrons, are enriched in conjugative plasmids. Notably, we report the discovery and characterization of a massive insertion sequence (IS)-associated AMR gene transfer network (245 combinations covering 59 AMR gene subtypes and 53 ISs) linking conjugative plasmids and phylogenetically distant pathogens, suggesting a general evolutionary mechanism for the horizontal transfer of AMR genes mediated by the interaction between conjugative plasmids and ISs. Moreover, our experimental results confirmed the importance of the observed interactions in aiding the horizontal transfer and expanding the genetic range of AMR genes within complex microbial communities. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Antimicrobial resistance; Horizontal gene transfer; Long-reads sequencing; Mobile genetic elements; Whole genome
语种	英语
scopus关键词	antibiotic resistance; article; class 1 integron; conjugative plasmid; gene insertion sequence; horizontal gene transfer; infectious agent; microbial community; nonhuman
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180721
作者单位	Environmental Microbiome Engineering and Biotechnology Laboratory, Department of Civil Engineering, University of Hong Kong, Hong Kong; Department of Biomedical Informatics, Harvard Medical School, Boston, MA 02115, United States; Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard T. H. Chan School of Public Health, Harvard University, Boston, MA 02115, United States; Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Department of Microbial Ecology, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, 1090, Austria
推荐引用方式 GB/T 7714	Che Y.,Yang Y.,Xu X.,et al. Conjugative plasmids interact with insertion sequences to shape the horizontal transfer of antimicrobial resistance genes[J],2021,118(6).
APA	Che Y..,Yang Y..,Xu X..,Brinda K..,Polz M.F..,...&Zhang T..(2021).Conjugative plasmids interact with insertion sequences to shape the horizontal transfer of antimicrobial resistance genes.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Che Y.,et al."Conjugative plasmids interact with insertion sequences to shape the horizontal transfer of antimicrobial resistance genes".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).