气候变化领域集成服务门户(CCPortal): Cross-talk of insulin-like peptides, juvenile hormone, and 20-hydroxyecdysone in regulation of metabolism in the mosquito aedes aegypti

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DOI	10.1073/pnas.2023470118
	Cross-talk of insulin-like peptides, juvenile hormone, and 20-hydroxyecdysone in regulation of metabolism in the mosquito aedes aegypti
	Ling L.; Raikhel A.S.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Female mosquitoes feed sequentially on carbohydrates (nectar) and proteins (blood) during each gonadotrophic cycle to become reproductively competent and effective disease vectors. Accordingly, metabolism is synchronized to support this reproductive cyclicity. However, regulatory pathways linking metabolism to reproductive cycles are not fully understood. Two key hormones, juvenile hormone (JH) and ecdysteroids (20-hydroxyecdysone, 20E, is the most active form) govern female mosquito reproduction. Aedes aegypti genome codes for eight insulin-like peptides (ILPs) that are critical for controlling metabolism. We examined the effects of the JH and 20E pathways on mosquito ILP expression to decipher regulation of metabolism in a reproducing female mosquito. Chromatin immunoprecipitation assays showed genomic interactions between ilp genes and the JH receptor, methoprene-tolerant, a transcription factor, Krüppel homolog 1 (Kr-h1), and two isoforms of the ecdysone response early gene, E74. The luciferase reporter assays showed that Kr-h1 activates ilps 2, 6, and 7, but represses ilps 4 and 5. The 20E pathway displayed the opposite effect in the regulation of ilps. E74B repressed ilps 2 and 6, while E74A activated ilps 4 and 5. Combining RNA interference, CRISPR gene tagging and enzyme-linked immunosorbent assay, we have shown that the JH and 20E regulate protein levels of all eight Ae. aegypti ILPs. Thus, we have established a regulatory axis between ILPs, JH, and 20E in coordination of metabolism during gonadotrophic cycles of Ae. aegypti. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	CRISPR-Cas9; Hormone; Insect; Insulin; Metabolism
语种	英语
scopus关键词	CRISPR associated endonuclease Cas9; ecdysone; ecdysone receptor; ecdysterone; insulin like peptide; juvenile hormone; kruppel like factor; Myc protein; peptides and proteins; relaxin 3; transcription factor FOXO; unclassified drug; adult; Aedes aegypti; Article; binding affinity; chromatin immunoprecipitation; clustered regularly interspaced short palindromic repeat; controlled study; down regulation; enzyme linked immunosorbent assay; female; female fertility; gene; gene activation; gene expression regulation; gene interaction; gene knockdown; gene mutation; gene silencing; ILP1 gene; ILP2 gene; ILP3 gene; ILP4 gene; ILP5 gene; ILP6 gene; ILP7 gene; ILP8 gene; luciferase assay; metabolic regulation; nonhuman; ovary cycle; priority journal; protein expression; protein function; protein localization; real time polymerase chain reaction; receptor cross-talk; RNA interference; upregulation; wild type
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180705
作者单位	Department of Entomology, University of California, Riverside, CA 92521, United States; Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, United States
推荐引用方式 GB/T 7714	Ling L.,Raikhel A.S.. Cross-talk of insulin-like peptides, juvenile hormone, and 20-hydroxyecdysone in regulation of metabolism in the mosquito aedes aegypti[J],2021,118(6).
APA	Ling L.,&Raikhel A.S..(2021).Cross-talk of insulin-like peptides, juvenile hormone, and 20-hydroxyecdysone in regulation of metabolism in the mosquito aedes aegypti.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Ling L.,et al."Cross-talk of insulin-like peptides, juvenile hormone, and 20-hydroxyecdysone in regulation of metabolism in the mosquito aedes aegypti".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).