气候变化领域集成服务门户(CCPortal): Reduced proinsecticide activation by cytochrome P450 confers coumaphos resistance in the major bee parasite varroa destructor

CCPortal

DOI	10.1073/pnas.2020380118
	Reduced proinsecticide activation by cytochrome P450 confers coumaphos resistance in the major bee parasite varroa destructor
	Vlogiannitis S.; Mavridis K.; Dermauw W.; Snoeck S.; Katsavou E.; Morou E.; Harizanis P.; Swevers L.; Hemingway J.; Feyereisen R.; van Leeuwen T.; Vontas J.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Varroa destructor is one of the main problems in modern beekeeping. Highly selective acaricides with low toxicity to bees are used internationally to control this mite. One of the key acaricides is the organophosphorus (OP) proinsecticide coumaphos, that becomes toxic after enzymatic activation inside Varroa. We show here that mites from the island Andros (AN-CR) exhibit high levels of coumaphos resistance. Resistance is not mediated by decreased coumaphos uptake, target-site resistance, or increased detoxification. Reduced proinsecticide activation by a cytochrome P450 enzyme was the main resistance mechanism, a powerful and rarely encountered evolutionary solution to insecticide selection pressure. After treatment with sublethal doses of [14C] coumaphos, susceptible mite extracts had substantial amounts of coroxon, the activated metabolite of coumaphos, while resistant mites had only trace amounts. This indicates a suppression of the P450 (CYP)-mediated activation step in the AN-CR mites. Bioassays with coroxon to bypass the activation step showed that resistance was dramatically reduced. There are 26 CYPs present in the V. destructor genome. Transcriptome analysis revealed overexpression in resistant mites of CYP4DP24 and underexpression of CYP3012A6 and CYP4EP4. RNA interference of CYP4EP4 in the susceptible population, to mimic underexpression seen in the resistant mites, prevented coumaphos activation and decreased coumaphos toxicity. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	CYPome; Honey bee; Pesticide; Resistance management; Selectivity
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180699
作者单位	Department of Crop Science, Agricultural University of Athens, Athens, GR-11855, Greece; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Heraklion, Crete, GR-700 13, Greece; Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, B-9000, Belgium; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food, Merelbeke, B-9820, Belgium; Department of Biology, University of Washington, Seattle, WA 98195, United States; Insect Molecular Genetics and Biotechnology, Institute of Biosciences and Applications, National Centre for Scientific Research “Demokritos,”, Athens, GR-153 10, Greece; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, L3 5QA, United Kingdom; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg C, Copenhagen, DK-1871, Denmark
推荐引用方式 GB/T 7714	Vlogiannitis S.,Mavridis K.,Dermauw W.,et al. Reduced proinsecticide activation by cytochrome P450 confers coumaphos resistance in the major bee parasite varroa destructor[J],2021,118(6).
APA	Vlogiannitis S..,Mavridis K..,Dermauw W..,Snoeck S..,Katsavou E..,...&Vontas J..(2021).Reduced proinsecticide activation by cytochrome P450 confers coumaphos resistance in the major bee parasite varroa destructor.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Vlogiannitis S.,et al."Reduced proinsecticide activation by cytochrome P450 confers coumaphos resistance in the major bee parasite varroa destructor".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).