气候变化领域集成服务门户(CCPortal): The evolution of red color vision is linked to coordinated rhodopsin tuning in lycaenid butterflies

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DOI	10.1073/pnas.2008986118
	The evolution of red color vision is linked to coordinated rhodopsin tuning in lycaenid butterflies
	Liénard M.A.; Bernard G.D.; Allen A.; Lassance J.-M.; Song S.; Childers R.R.; Yu N.; Ye D.; Stephenson A.; Valencia-Montoya W.A.; Salzman S.; Whitaker M.R.L.; Calonje M.; Zhang F.; Pierce N.E.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Color vision has evolved multiple times in both vertebrates and invertebrates and is largely determined by the number and variation in spectral sensitivities of distinct opsin subclasses. However, because of the difficulty of expressing long-wavelength (LW) invertebrate opsins in vitro, our understanding of the molecular basis of functional shifts in opsin spectral sensitivities has been biased toward research primarily in vertebrates. This has restricted our ability to address whether invertebrate Gq protein-coupled opsins function in a novel or convergent way compared to vertebrate Gt opsins. Here we develop a robust heterologous expression system to purify invertebrate rhodopsins, identify specific amino acid changes responsible for adaptive spectral tuning, and pinpoint how molecular variation in invertebrate opsins underlie wavelength sensitivity shifts that enhance visual perception. By combining functional and optophysiological approaches, we disentangle the relative contributions of lateral filtering pigments from red-shifted LW and blue short-wavelength opsins expressed in distinct photoreceptor cells of individual ommatidia. We use in situ hybridization to visualize six ommatidial classes in the compound eye of a lycaenid butterfly with a four-opsin visual system. We show experimentally that certain key tuning residues underlying green spectral shifts in blue opsin paralogs have evolved repeatedly among short-wavelength opsin lineages. Taken together, our results demonstrate the interplay between regulatory and adaptive evolution at multiple Gq opsin loci, as well as how coordinated spectral shifts in LW and blue opsins can act together to enhance insect spectral sensitivity at blue and red wavelengths for visual performance adaptation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Ecological adaptation; Insects; Molecular evolution; Spectral sensitivity; Visual system
语种	英语
scopus关键词	rhodopsin; animal experiment; Article; butterfly; color vision; controlled study; evolutionary adaptation; gene; gene duplication; gene locus; genetic variability; heterologous expression; in situ hybridization; nonhuman; perceptive threshold; photoreceptor cell; priority journal; process development; protein expression; protein function; protein purification; rhodopsin gene; sensitivity analysis; visual acuity; visual adaptation; visual system
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180697
作者单位	Broad Institute of MIT, Harvard University, Cambridge, MA 02142, United States; Department of Organismic and Evolutionary Biology, Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, United States; Department of Electrical and Computer Engineering, University of Washington, Seattle, WA 98195, United States; Department of Applied Physics and Applied Mathematics, Columbia University, New York, NY 10027, United States; Montgomery Botanical Center, Miami, FL 33156, United States; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Howard Hughes Medical Institute, Cambridge, MA 02139, United States; Department of Biology, Lund University, Lund, 22362, Sweden; Department of Ecology and Evolutio...
推荐引用方式 GB/T 7714	Liénard M.A.,Bernard G.D.,Allen A.,et al. The evolution of red color vision is linked to coordinated rhodopsin tuning in lycaenid butterflies[J],2021,118(6).
APA	Liénard M.A..,Bernard G.D..,Allen A..,Lassance J.-M..,Song S..,...&Pierce N.E..(2021).The evolution of red color vision is linked to coordinated rhodopsin tuning in lycaenid butterflies.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Liénard M.A.,et al."The evolution of red color vision is linked to coordinated rhodopsin tuning in lycaenid butterflies".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).