气候变化领域集成服务门户(CCPortal): Cell dispersal by localized degradation of a chemoattractant

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DOI	10.1073/pnas.2008126118
	Cell dispersal by localized degradation of a chemoattractant
	Karmakar R.; Tyree T.; Gomer R.H.; Rappel W.-J.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Chemotaxis, the guided motion of cells by chemical gradients, plays a crucial role in many biological processes. In the social amoeba Dictyostelium discoideum, chemotaxis is critical for the formation of cell aggregates during starvation. The cells in these aggregates generate a pulse of the chemoattractant, cyclic adenosine 3',5'-monophosphate (cAMP), every 6 min to 10 min, resulting in surrounding cells moving toward the aggregate. In addition to periodic pulses of cAMP, the cells also secrete phosphodiesterase (PDE), which degrades cAMP and prevents the accumulation of the chemoattractant. Here we show that small aggregates of Dictyostelium can disperse, with cells moving away from instead of toward the aggregate. This surprising behavior often exhibited oscillatory cycles of motion toward and away from the aggregate. Furthermore, the onset of outward cell motion was associated with a doubling of the cAMP signaling period. Computational modeling suggests that this dispersal arises from a competition between secreted cAMP and PDE, creating a cAMP gradient that is directed away from the aggregate, resulting in outward cell motion. The model was able to predict the effect of PDE inhibition as well as global addition of exogenous PDE, and these predictions were subsequently verified in experiments. These results suggest that localized degradation of a chemoattractant is a mechanism for morphogenesis. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Chemotaxis; Dictyostelium discoideum; Dispersal; Repulsion
语种	英语
scopus关键词	chemoattractant; cyclic AMP; animal dispersal; Article; cell motion; computer model; controlled study; Dictyostelium discoideum; enzyme degradation; morphogenesis; nonhuman; priority journal; signal transduction
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180689
作者单位	Department of Physics, University of California San Diego, San Diego, CA 92093, United States; Department of Biology, Texas A&M University, College Station, TX 77843, United States
推荐引用方式 GB/T 7714	Karmakar R.,Tyree T.,Gomer R.H.,et al. Cell dispersal by localized degradation of a chemoattractant[J],2021,118(6).
APA	Karmakar R.,Tyree T.,Gomer R.H.,&Rappel W.-J..(2021).Cell dispersal by localized degradation of a chemoattractant.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Karmakar R.,et al."Cell dispersal by localized degradation of a chemoattractant".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).