气候变化领域集成服务门户(CCPortal): A novel stabilization mechanism for the type VI secretion system sheath

CCPortal

DOI	10.1073/pnas.2008500118
	A novel stabilization mechanism for the type VI secretion system sheath
	Bernal P.; Christopher D. Furniss R.; Fecht S.; Leung R.C.Y.; Spiga L.; Mavridou D.A.I.; Filloux A.
发表日期	2021
ISSN	00278424
卷号	118 期号:7
英文摘要	The type VI secretion system (T6SS) is a phage-derived contractile nanomachine primarily involved in interbacterial competition. Its pivotal component, TssA, is indispensable for the assembly of the T6SS sheath structure, the contraction of which propels a payload of effector proteins into neighboring cells. Despite their key function, TssA proteins exhibit unexpected diversity and exist in two major forms, a short form (TssAS) and a long form (TssAL). While TssAL proteins interact with a partner, called TagA, to anchor the distal end of the extended sheath, the mechanism for the stabilization of TssAS-containing T6SSs remains unknown. Here we discover a class of structural components that interact with short TssA proteins and contribute to T6SS assembly by stabilizing the polymerizing sheath from the baseplate. We demonstrate that the presence of these components is important for full sheath extension and optimal firing. Moreover, we show that the pairing of each form of TssA with a different class of sheath stabilization proteins results in T6SS apparatuses that either reside in the cell for some time or fire immediately after sheath extension. We propose that this diversity in firing dynamics could contribute to the specialization of the T6SS to suit bacterial lifestyles in diverse environmental niches. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Type VI secretion system \| TssA \| TagB \| sheath stabilization \| Pseudomonas
语种	英语
scopus关键词	article; lifestyle; nonhuman; Pseudomonas; specialization; type VI secretion system
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180648
作者单位	MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, London, SW7 2AZ, United Kingdom; Department of Biology, Faculty of Sciences, Universidad Autónoma de Madrid, Madrid, 28049, Spain; Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Seville, 41012, Spain; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, United States
推荐引用方式 GB/T 7714	Bernal P.,Christopher D. Furniss R.,Fecht S.,et al. A novel stabilization mechanism for the type VI secretion system sheath[J],2021,118(7).
APA	Bernal P..,Christopher D. Furniss R..,Fecht S..,Leung R.C.Y..,Spiga L..,...&Filloux A..(2021).A novel stabilization mechanism for the type VI secretion system sheath.Proceedings of the National Academy of Sciences of the United States of America,118(7).
MLA	Bernal P.,et al."A novel stabilization mechanism for the type VI secretion system sheath".Proceedings of the National Academy of Sciences of the United States of America 118.7(2021).