气候变化领域集成服务门户(CCPortal): The Myc-associated zinc finger protein (MAZ) works together with CTCF to control cohesin positioning and genome organization

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DOI	10.1073/pnas.2023127118
	The Myc-associated zinc finger protein (MAZ) works together with CTCF to control cohesin positioning and genome organization
	Xiao T.; Li X.; Felsenfeld G.
发表日期	2021
ISSN	00278424
卷号	118 期号:7
英文摘要	The Myc-associated zinc finger protein (MAZ) is often found at genomic binding sites adjacent to CTCF, a protein which affects large-scale genome organization through its interaction with cohesin. We show here that, like CTCF, MAZ physically interacts with a cohesin subunit and can arrest cohesin sliding independently of CTCF. It also shares with CTCF the ability to independently pause the elongating form of RNA polymerase II, and consequently affects RNA alternative splicing. CTCF/MAZ double sites are more effective at sequestering cohesin than sites occupied only by CTCF. Furthermore, depletion of CTCF results in preferential loss of CTCF from sites not occupied by MAZ. In an assay for insulation activity like that used for CTCF, binding of MAZ to sites between an enhancer and promoter results in down-regulation of reporter gene expression, supporting a role for MAZ as an insulator protein. Hi-C analysis of the effect of MAZ depletion on genome organization shows that local interactions within topologically associated domains (TADs) are disrupted, as well as contacts that establish the boundaries of individual TADs. We conclude that MAZ augments the action of CTCF in organizing the genome, but also shares properties with CTCF that allow it to act independently. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	MAZ \| CTCF \| cohesin arrest \| insulation \| RNA Pol II pausing
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180646
作者单位	Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892-0540, United States
推荐引用方式 GB/T 7714	Xiao T.,Li X.,Felsenfeld G.. The Myc-associated zinc finger protein (MAZ) works together with CTCF to control cohesin positioning and genome organization[J],2021,118(7).
APA	Xiao T.,Li X.,&Felsenfeld G..(2021).The Myc-associated zinc finger protein (MAZ) works together with CTCF to control cohesin positioning and genome organization.Proceedings of the National Academy of Sciences of the United States of America,118(7).
MLA	Xiao T.,et al."The Myc-associated zinc finger protein (MAZ) works together with CTCF to control cohesin positioning and genome organization".Proceedings of the National Academy of Sciences of the United States of America 118.7(2021).