气候变化领域集成服务门户(CCPortal): Local translation in nuclear condensate amyloid bodies

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DOI	10.1073/pnas.2014457118
	Local translation in nuclear condensate amyloid bodies
	Theodoridis P.R.; Bokros M.; Marijan D.; Balukoff N.C.; Wang D.; Kirk C.C.; Budine T.D.; Goldsmith H.D.; Wang M.; Audas T.E.; Lee S.
发表日期	2021
ISSN	00278424
卷号	118 期号:7
英文摘要	Biomolecular condensates concentrate molecules to facilitate basic biochemical processes, including transcription and DNA replication. While liquid-like condensates have been ascribed various functions, solid-like condensates are generally thought of as amorphous sites of protein storage. Here, we show that solid-like amyloid bodies coordinate local nuclear protein synthesis (LNPS) during stress. On stimulus, translationally active ribosomes accumulate along fiber-like assemblies that characterize amyloid bodies. Mass spectrometry analysis identified regulatory ribosomal proteins and translation factors that relocalize from the cytoplasm to amyloid bodies to sustain LNPS. These amyloidogenic compartments are enriched in newly transcribed messenger RNA by Heat Shock Factor 1 (HSF1). Depletion of stress-induced ribosomal intergenic spacer noncoding RNA (rIGSRNA) that constructs amyloid bodies prevents recruitment of the nuclear protein synthesis machinery, abolishes LNPS, and impairs the nuclear HSF1 response. We propose that amyloid bodies support local nuclear translation during stress and that solid-like condensates can facilitate complex biochemical reactions as their liquid counterparts can. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Long noncoding RNA \| hypoxia \| acidosis \| HSR \| Hsp70
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180613
作者单位	Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, United States; Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL 33136, United States; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
推荐引用方式 GB/T 7714	Theodoridis P.R.,Bokros M.,Marijan D.,et al. Local translation in nuclear condensate amyloid bodies[J],2021,118(7).
APA	Theodoridis P.R..,Bokros M..,Marijan D..,Balukoff N.C..,Wang D..,...&Lee S..(2021).Local translation in nuclear condensate amyloid bodies.Proceedings of the National Academy of Sciences of the United States of America,118(7).
MLA	Theodoridis P.R.,et al."Local translation in nuclear condensate amyloid bodies".Proceedings of the National Academy of Sciences of the United States of America 118.7(2021).