气候变化领域集成服务门户(CCPortal): Mapping proteolytic neo-N termini at the surface of living cells

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DOI	10.1073/pnas.2018809118
	Mapping proteolytic neo-N termini at the surface of living cells
	Weeks A.M.; Byrnes J.R.; Lui I.; Wells J.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	N terminomics is a powerful strategy for profiling proteolytic neoN termini, but its application to cell surface proteolysis has been limited by the low relative abundance of plasma membrane proteins. Here we apply plasma membrane-targeted subtiligase variants (subtiligase-TM) to efficiently and specifically capture cell surface N termini in live cells. Using this approach, we sequenced 807 cell surface N termini and quantified changes in their abundance in response to stimuli that induce proteolytic remodeling of the cell surface proteome. To facilitate exploration of our datasets, we developed a web-accessible Atlas of Subtiligase-Captured Extracellular N Termini (ASCENT; http://wellslab.org/ascent). This technology will facilitate greater understanding of extracellular protease biology and reveal neo-N termini biomarkers and targets in disease. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	N terminomics; Proteolysis; Proteomics
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180596
作者单位	Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, United States; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, United States; Department of Biochemistry, University of Wisconsin–Madison, Madison, WI 53706, United States
推荐引用方式 GB/T 7714	Weeks A.M.,Byrnes J.R.,Lui I.,et al. Mapping proteolytic neo-N termini at the surface of living cells[J],2021,118(8).
APA	Weeks A.M.,Byrnes J.R.,Lui I.,&Wells J.A..(2021).Mapping proteolytic neo-N termini at the surface of living cells.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Weeks A.M.,et al."Mapping proteolytic neo-N termini at the surface of living cells".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).