气候变化领域集成服务门户(CCPortal): Transiently structured head domains control intermediate filament assembly

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DOI	10.1073/pnas.2022121118
	Transiently structured head domains control intermediate filament assembly
	Zhou X.; Lin Y.; Kato M.; Mori E.; Liszczak G.; Sutherland L.; Sysoev V.O.; Murray D.T.; Tycko R.; McKnight S.L.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	Low complexity (LC) head domains 92 and 108 residues in length are, respectively, required for assembly of neurofilament light (NFL) and desmin intermediate filaments (IFs). As studied in isolation, these IF head domains interconvert between states of conformational disorder and labile, β-strand–enriched polymers. Solid-state NMR (ss-NMR) spectroscopic studies of NFL and desmin head domain polymers reveal spectral patterns consistent with structural order. A combination of intein chemistry and segmental isotope labeling allowed preparation of fully assembled NFL and desmin IFs that could also be studied by ss-NMR. Assembled IFs revealed spectra overlapping with those observed for β-strand–enriched polymers formed from the isolated NFL and desmin head domains. Phosphorylation and disease-causing mutations reciprocally alter NFL and desmin head domain self-association yet commonly impede IF assembly. These observations show how facultative structural assembly of LC domains via labile, β-strand–enriched self-interactions may broadly influence cell morphology. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	In situ structural analysis; Intermediate filaments; Labile cross-β structures; Low complexity domains; Phase separation
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180586
作者单位	Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States; Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan; Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, United States; Department of Future Basic Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, Japan; Department of Chemistry, University of California, Davis, CA 95616, United States; Tsinghua-Peking Center for Life Science, IDG/McGovern Institute for Brain Research, School of Life Sciences, Tsinghua University, Beijing, 100084, China
推荐引用方式 GB/T 7714	Zhou X.,Lin Y.,Kato M.,et al. Transiently structured head domains control intermediate filament assembly[J],2021,118(8).
APA	Zhou X..,Lin Y..,Kato M..,Mori E..,Liszczak G..,...&McKnight S.L..(2021).Transiently structured head domains control intermediate filament assembly.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Zhou X.,et al."Transiently structured head domains control intermediate filament assembly".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).