气候变化领域集成服务门户(CCPortal): The male germline-specific protein MAPS is indispensable for pachynema progression and fertility

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DOI	10.1073/pnas.2025421118
	The male germline-specific protein MAPS is indispensable for pachynema progression and fertility
	Li M.; Zheng J.; Li G.; Lin Z.; Li D.; Liu D.; Feng H.; Cao D.; Ng E.H.Y.; Li R.H.W.; Han C.; Yeung W.S.B.; Chow L.T.; Wang H.; Liu K.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	Meiosis is a specialized cell division that creates haploid germ cells from diploid progenitors. Through differential RNA expression analyses, we previously identified a number of mouse genes that were dramatically elevated in spermatocytes, relative to their very low expression in spermatogonia and somatic organs. Here, we investigated in detail 1700102P08Rik, one of these genes, and independently conclude that it encodes a male germline-specific protein, in agreement with a recent report. We demonstrated that it is essential for pachynema progression in spermatocytes and named it male pachynema-specific (MAPS) protein. Mice lacking Maps (Maps−/−) suffered from pachytene arrest and spermatocyte death, leading to male infertility, whereas female fertility was not affected. Interestingly, pubertal Maps−/− spermatocytes were arrested at early pachytene stage, accompanied by defects in DNA double-strand break (DSB) repair, crossover formation, and XY body formation. In contrast, adult Maps−/− spermatocytes only exhibited partially defective crossover but nonetheless were delayed or failed in progression from early to mid- and late pachytene stage, resulting in cell death. Furthermore, we report a significant transcriptional dysregulation in autosomes and XY chromosomes in both pubertal and adult Maps−/− pachytene spermatocytes, including failed meiotic sex chromosome inactivation (MSCI). Further experiments revealed that MAPS overexpression in vitro dramatically decreased the ubiquitination levels of cellular proteins. Conversely, in Maps−/− pachytene cells, protein ubiquitination was dramatically increased, likely contributing to the large-scale disruption in gene expression in pachytene cells. Thus, MAPS is a protein essential for pachynema progression in male mice, possibly in mammals in general. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Male germline-specific protein; MAPS; Meiosis prophase I; Pachynema progression
语种	英语
scopus关键词	cell protein; male pachynema specific protein; unclassified drug; adult; adulthood; animal cell; animal experiment; animal model; Article; autosome; cell death; cellular distribution; chromosome inactivation; chromosome pairing; controlled study; crossing over; double strand break repair; female; female infertility; fetus; gene disruption; gene expression regulation; gene overexpression; germline stem cell; in vitro study; male; male fertility; male infertility; meiosis; meiotic sex chromosome inactivation; mouse; nonhuman; pachytene; priority journal; prophase; protein localization; puberty; sex chromosome; testicular cell line; transcription regulation; ubiquitination; X chromosome; Y chromosome
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180583
作者单位	Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, University of Hong Kong–Shenzhen Hospital, Shenzhen, Guangdong, 518053, China; Department of Obstetrics and Gynecology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, Hong Kong; Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294-0024, United States; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, 100049, China
推荐引用方式 GB/T 7714	Li M.,Zheng J.,Li G.,et al. The male germline-specific protein MAPS is indispensable for pachynema progression and fertility[J],2021,118(8).
APA	Li M..,Zheng J..,Li G..,Lin Z..,Li D..,...&Liu K..(2021).The male germline-specific protein MAPS is indispensable for pachynema progression and fertility.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Li M.,et al."The male germline-specific protein MAPS is indispensable for pachynema progression and fertility".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).