气候变化领域集成服务门户(CCPortal): The DUF1013 protein TrcR tracks with RNA polymerase to control the bacterial cell cycle and protect against antibiotics

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DOI	10.1073/pnas.2010357118
	The DUF1013 protein TrcR tracks with RNA polymerase to control the bacterial cell cycle and protect against antibiotics
	Delaby M.; Varesio L.M.; Degeorges L.; Crosson S.; Viollier P.H.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	How DNA-dependent RNA polymerase (RNAP) acts on bacterial cell cycle progression during transcription elongation is poorly investigated. A forward genetic selection for Caulobacter crescentus cell cycle mutants unearthed the uncharacterized DUF1013 protein (TrcR, transcriptional cell cycle regulator). TrcR promotes the accumulation of the essential cell cycle transcriptional activator CtrA in late S-phase but also affects transcription at a global level to protect cells from the quinolone antibiotic nalidixic acid that induces a multidrug efflux pump and from the RNAP inhibitor rifampicin that blocks transcription elongation. We show that TrcR associates with promoters and coding sequences in vivo in a rifampicin-dependent manner and that it interacts physically and genetically with RNAP. We show that TrcR function and its RNAP-dependent chromatin recruitment are conserved in symbiotic Sinorhizobium sp. and pathogenic Brucella spp. Thus, TrcR represents a hitherto unknown antibiotic target and the founding member of the DUF1013 family, an uncharacterized class of transcriptional regulators that track with RNAP during the elongation phase to promote transcription during the cell cycle. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Brucella; Caulobacter; RNA polymerase; Transcription; TrcR
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180564
作者单位	Department of Microbiology and Molecular Medicine, Faculty of Medicine, Centre Médical Universitaire, University of Geneva, Genève 4, 1211, Switzerland; Committee on Microbiology, University of Chicago, Chicago, IL 60637, United States; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States
推荐引用方式 GB/T 7714	Delaby M.,Varesio L.M.,Degeorges L.,et al. The DUF1013 protein TrcR tracks with RNA polymerase to control the bacterial cell cycle and protect against antibiotics[J],2021,118(8).
APA	Delaby M.,Varesio L.M.,Degeorges L.,Crosson S.,&Viollier P.H..(2021).The DUF1013 protein TrcR tracks with RNA polymerase to control the bacterial cell cycle and protect against antibiotics.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Delaby M.,et al."The DUF1013 protein TrcR tracks with RNA polymerase to control the bacterial cell cycle and protect against antibiotics".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).