气候变化领域集成服务门户(CCPortal): Structured sequences emerge from random pool when replicated by templated ligation

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DOI	10.1073/pnas.2018830118
	Structured sequences emerge from random pool when replicated by templated ligation
	Kudella P.W.; Tkachenko A.V.; Salditt A.; Maslov S.; Braun D.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	The central question in the origin of life is to understand how structure can emerge from randomness. The Eigen theory of replication states, for sequences that are copied one base at a time, that the replication fidelity has to surpass an error threshold to avoid that replicated specific sequences become random because of the incorporated replication errors [M. Eigen, Naturwissenschaften 58 (10), 465–523 (1971)]. Here, we showed that linking short oligomers from a random sequence pool in a templated ligation reaction reduced the sequence space of product strands. We started from 12-mer oligonucleotides with two bases in all possible combinations and triggered enzymatic ligation under temperature cycles. Surprisingly, we found the robust creation of long, highly structured sequences with low entropy. At the ligation site, complementary and alternating sequence patterns developed. However, between the ligation sites, we found either an A-rich or a T-rich sequence within a single oligonucleotide. Our modeling suggests that avoidance of hairpins was the likely cause for these two complementary sequence pools. What emerged was a network of complementary sequences that acted both as templates and substrates of the reaction. This self-selecting ligation reaction could be restarted by only a few majority sequences. The findings showed that replication by random templated ligation from a random sequence input will lead to a highly structured, long, and nonrandom sequence pool. This is a favorable starting point for a subsequent Darwinian evolution searching for higher catalytic functions in an RNA world scenario. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Darwinian evolution; DNA replication; Origin of life; Sequence entropy; Templated ligation
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180559
作者单位	Systems Biophysics and Center for NanoScience, Ludwigs-Maximilian-Universität München, Munich, 80799, Germany; Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, NY 11973, United States; Department of Bioengineering, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States
推荐引用方式 GB/T 7714	Kudella P.W.,Tkachenko A.V.,Salditt A.,et al. Structured sequences emerge from random pool when replicated by templated ligation[J],2021,118(8).
APA	Kudella P.W.,Tkachenko A.V.,Salditt A.,Maslov S.,&Braun D..(2021).Structured sequences emerge from random pool when replicated by templated ligation.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Kudella P.W.,et al."Structured sequences emerge from random pool when replicated by templated ligation".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).