气候变化领域集成服务门户(CCPortal): Competitive binding of independent extension and retraction motors explains the quantitative dynamics of type IV pili

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DOI	10.1073/pnas.2014926118
	Competitive binding of independent extension and retraction motors explains the quantitative dynamics of type IV pili
	Koch M.D.; Fei C.; Wingreen N.S.; Shaevitz J.W.; Gitai Z.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	Type IV pili (TFP) function through cycles of extension and retraction. The coordination of these cycles remains mysterious due to a lack of quantitative measurements of multiple features of TFP dynamics. Here, we fluorescently label TFP in the pathogen Pseudomonas aeruginosa and track full extension and retraction cycles of individual filaments. Polymerization and depolymerization dynamics are stochastic; TFP are made at random times and extend, pause, and retract for random lengths of time. TFP can also pause for extended periods between two extension or two retraction events in both wild-type cells and a slowly retracting PilT mutant. We developed a biophysical model based on the stochastic binding of two dedicated extension and retraction motors to the same pilus machine that predicts the observed features of the data with no free parameters. We show that only a model in which both motors stochastically bind and unbind to the pilus machine independent of the piliation state of the machine quantitatively explains the experimentally observed pilus production rate. In experimental support of this model, we show that the abundance of the retraction motor dictates the pilus production rate and that PilT is bound to pilus machines even in their unpiliated state. Together, the strong quantitative agreement of our model with a variety of experiments suggests that the entire repetitive cycle of pilus extension and retraction is coordinated by the competition of stochastic motor binding to the pilus machine, and that the retraction motor is the major throttle for pilus production. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Competitive binding; Molecular motor; Pilus dynamics; Type IV pili
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180555
作者单位	Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08540, United States; Department of Molecular Biology, Princeton University, Princeton, NJ 08540, United States; Joseph Henry Laboratories of Physics, Princeton University, Princeton, NJ 08540, United States
推荐引用方式 GB/T 7714	Koch M.D.,Fei C.,Wingreen N.S.,et al. Competitive binding of independent extension and retraction motors explains the quantitative dynamics of type IV pili[J],2021,118(8).
APA	Koch M.D.,Fei C.,Wingreen N.S.,Shaevitz J.W.,&Gitai Z..(2021).Competitive binding of independent extension and retraction motors explains the quantitative dynamics of type IV pili.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Koch M.D.,et al."Competitive binding of independent extension and retraction motors explains the quantitative dynamics of type IV pili".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).