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DOI	10.1073/pnas.2007192118
	Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences
	Li W.-C.; Lee C.-Y.; Lan W.-H.; Woo T.-T.; Liu H.-C.; Yeh H.-Y.; Chang H.-Y.; Chuang Y.-C.; Chen C.-Y.; Chuang C.-N.; Chen C.-L.; Hsueh Y.-P.; Li H.-W.; Chi P.; Wang T.-F.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombination during meiosis. However, some eukaryotes have lost Dmc1. Given that mammalian and yeast Saccharomyces cerevisiae (Sc) Dmc1 have been shown to stabilize recombination intermediates containing mismatches better than Rad51, we used the Pezizomycotina filamentous fungus Trichoderma reesei to address if and how Rad51-only eukaryotes conduct interhomolog recombination in zygotes with high sequence heterogeneity. We applied multidisciplinary approaches (next- and third-generation sequencing technology, genetics, cytology, bioinformatics, biochemistry, and single-molecule biophysics) to show that T. reesei Rad51 (TrRad51) is indispensable for interhomolog recombination during meiosis and, like ScDmc1, TrRad51 possesses better mismatch tolerance than ScRad51 during homologous recombination. Our results also indicate that the ancestral TrRad51 evolved to acquire ScDmc1-like properties by creating multiple structural variations, including via amino acid residues in the L1 and L2 DNA-binding loops. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Dmc1; Homologous recombination; Meiosis; Rad51; Trichoderma
语种	英语
scopus关键词	amino acid; bacterial protein; Dmc1 protein; Rad51 protein; unclassified drug; Article; base mispairing; biochemistry; bioinformatics; biophysics; controlled study; cytology; DNA binding; DNA helix; double strand break repair; gene; gene sequence; genetic variability; homologous recombination; Hypocrea jecorina; MAT1 1 gene; meiosis; nonhuman; priority journal; protein polymorphism; Saccharomyces cerevisiae; vegetative growth; zygote
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180541
作者单位	Taiwan International Graduate Program in Molecular and Cellular Biology, Academia Sinica, Taipei, 11529, Taiwan; Institute of Life Sciences, National Defense Medical Center, Taipei, 11490, Taiwan; Institute of Molecular Biology, Academia Sinica, Taipei, 11529, Taiwan; Institute of Biochemical Sciences, National Taiwan University, Taipei, 10617, Taiwan; Department of Chemistry, National Taiwan University, Taipei, 10617, Taiwan; Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan
推荐引用方式 GB/T 7714	Li W.-C.,Lee C.-Y.,Lan W.-H.,et al. Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences[J],2021,118(8).
APA	Li W.-C..,Lee C.-Y..,Lan W.-H..,Woo T.-T..,Liu H.-C..,...&Wang T.-F..(2021).Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Li W.-C.,et al."Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).