气候变化领域集成服务门户(CCPortal): A de novo strategy to develop NIR precipitating fluorochrome for long-term in situ cell membrane bioimaging

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DOI	10.1073/pnas.2018033118
	A de novo strategy to develop NIR precipitating fluorochrome for long-term in situ cell membrane bioimaging
	Li K.; Lyu Y.; Huang Y.; Xu S.; Liu H.-W.; Chen L.; Ren T.-B.; Xiong M.; Huan S.; Yuan L.; Zhang X.-B.; Tan W.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	Cell membrane–targeted bioimaging is a prerequisite for studying the roles of membrane-associated biomolecules in various physiological and pathological processes. However, long-term in situ bioimaging on the cell membrane with conventional fluorescent probes leads to diffusion into cells from the membrane surface. Therefore, we herein proposed a de novo strategy to construct an antidiffusion probe by integrating a fluorochrome characterized by strong hydrophobicity and low lipophilicity, with an enzyme substrate to meet this challenge. This precipitating fluorochrome HYPQ was designed by conjugating the traditionally strong hydrophobic solid-state fluorochrome 6-chloro–2-(2-hydroxyphenyl) quinazolin-4(3H)-one (HPQ) with a 2-(2-methyl–4H-chromen–4-ylidene) malononitrile group to obtain closer stacking to lower lipophilicity and elongate emission to the far-red to near-infrared wavelength. As proof-of-concept, the membrane-associated enzyme γ-glutamyltranspeptidase (GGT) was selected as a model enzyme to design the antidiffusion probe HYPQG. Then, benefiting from the precipitating and stable signal properties of HYPQ, in situ imaging of GGT on the membrane was successfully realized. Moreover, after HYPQG was activated by GGT, the fluorescence signal on the cell membrane remained unchanged, with incubation time even extending to 6 h, which is significant for in situ monitoring of enzymatic activity. In vivo testing subsequently showed that the tumor region could be accurately defined by this probe after long-term in situ imaging of tumor-bearing mice. The excellent performance of HYPQ indicates that it may be an ideal alternative for constructing universal antidiffusion fluorescent probes, potentially providing an efficient tool for accurate imaging-guided surgery in the future. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Antidiffusion probe; Cell membrane–targeted bioimaging; In vivo bioimaging; Long-term in situ imaging; Precipitating fluorochrome
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180540
作者单位	Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China; Key Laboratory of Analysis and Detection Technology for Food Safety of the Ministry of Education, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350002, China
推荐引用方式 GB/T 7714	Li K.,Lyu Y.,Huang Y.,et al. A de novo strategy to develop NIR precipitating fluorochrome for long-term in situ cell membrane bioimaging[J],2021,118(8).
APA	Li K..,Lyu Y..,Huang Y..,Xu S..,Liu H.-W..,...&Tan W..(2021).A de novo strategy to develop NIR precipitating fluorochrome for long-term in situ cell membrane bioimaging.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Li K.,et al."A de novo strategy to develop NIR precipitating fluorochrome for long-term in situ cell membrane bioimaging".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).