气候变化领域集成服务门户(CCPortal): Identification of a nuclear receptor/coactivator developmental signaling pathway in the nematode parasite Strongyloides stercoralis

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DOI	10.1073/pnas.2021864118
	Identification of a nuclear receptor/coactivator developmental signaling pathway in the nematode parasite Strongyloides stercoralis
	Cheong M.C.; Wang Z.; Jaleta T.G.; Li X.; Lok J.B.; Kliewer S.A.; Mangelsdorf D.J.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	DAF-12 is nematode-specific nuclear receptor that has been proposed to govern development of the infectious stage of parasitic species, including Strongyloides stercoralis. Here, we identified a parasite-specific coactivator, called DAF-12 interacting protein-1 (DIP-1), that is required for DAF-12 ligand-dependent transcriptional activity. DIP-1 is found only in Strongyloides spp. and selectively interacts with DAF-12 through an atypical receptor binding motif. Using CRISPR/Cas9-directed mutagenesis, we demonstrate that DAF-12 is required for the requisite developmental arrest and the ligand-dependent reactivation of infectious S. stercoralis infective third-stage larvae, and that these effects require the DIP-1 coactivator. These studies reveal the existence of a distinct nuclear receptor/coactivator signaling pathway that governs parasite development. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Coactivator; DAF-12; Nematode parasite; Nuclear receptor; Strongyloides stercoralis
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180534
作者单位	Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States; HHMI, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States
推荐引用方式 GB/T 7714	Cheong M.C.,Wang Z.,Jaleta T.G.,et al. Identification of a nuclear receptor/coactivator developmental signaling pathway in the nematode parasite Strongyloides stercoralis[J],2021,118(8).
APA	Cheong M.C..,Wang Z..,Jaleta T.G..,Li X..,Lok J.B..,...&Mangelsdorf D.J..(2021).Identification of a nuclear receptor/coactivator developmental signaling pathway in the nematode parasite Strongyloides stercoralis.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Cheong M.C.,et al."Identification of a nuclear receptor/coactivator developmental signaling pathway in the nematode parasite Strongyloides stercoralis".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).