气候变化领域集成服务门户(CCPortal): A specific basal body linker protein provides the connection function for basal body inheritance in trypanosomes

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DOI	10.1073/pnas.2014040118
	A specific basal body linker protein provides the connection function for basal body inheritance in trypanosomes
	Lai D.-H.; Moreira-Leite F.; Xu Z.-S.; Yang J.; Gull K.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	Centrioles and basal bodies (CBBs) are found in physically linked pairs, and in mammalian cells intercentriole connections (G1–G2 tether and S–M linker) regulate centriole duplication and function. In trypanosomes BBs are not associated with the spindle and function in flagellum/cilia nucleation with an additional role in mitochondrial genome (kinetoplast DNA [kDNA]) segregation. Here, we describe BBLP, a BB/pro-BB (pBB) linker protein in Trypanosoma brucei predicted to be a large coiled-coil protein conserved in the kinetoplastida. Colocalization with the centriole marker SAS6 showed that BBLP localizes between the BB/pBB pair, throughout the cell cycle, with a stronger signal in the old flagellum BB/pBB pair. Importantly, RNA interference (RNAi) depletion of BBLP leads to a conspicuous splitting of the BB/pBB pair associated only with the new flagellum. BBLP RNAi is lethal in the bloodstream form of the parasite and perturbs mitochondrial kDNA inheritance. Immunogold labeling confirmed that BBLP is localized to a cytoskeletal component of the BB/pBB linker, and tagged protein induction showed that BBLP is incorporated de novo in both new and old flagella BB pairs of dividing cells. We show that the two aspects of CBB disengagement—loss of orthogonal orientation and ability to separate and move apart—are consistent but separable events in evolutionarily diverse cells and we provide a unifying model explaining centriole/BB linkage differences between such cells. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Basal bodies; BBLP; Cell cycle; Centrioles; Linker
语种	英语
scopus关键词	article; cell cycle; centriole; flagellum; immunogold labeling; inheritance; kinetosome; mitochondrion; nonhuman; parasite; protein function; protein induction; RNA interference; Trypanosoma brucei
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180531
作者单位	Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China; Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, OX3 0BP, United Kingdom; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, United Kingdom
推荐引用方式 GB/T 7714	Lai D.-H.,Moreira-Leite F.,Xu Z.-S.,et al. A specific basal body linker protein provides the connection function for basal body inheritance in trypanosomes[J],2021,118(8).
APA	Lai D.-H.,Moreira-Leite F.,Xu Z.-S.,Yang J.,&Gull K..(2021).A specific basal body linker protein provides the connection function for basal body inheritance in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Lai D.-H.,et al."A specific basal body linker protein provides the connection function for basal body inheritance in trypanosomes".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).