气候变化领域集成服务门户(CCPortal): Nile Red fluorescence spectroscopy reports early physicochemical changes in myelin with high sensitivity

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DOI	10.1073/pnas.2016897118
	Nile Red fluorescence spectroscopy reports early physicochemical changes in myelin with high sensitivity
	Teo W.; Caprariello A.V.; Morgan M.L.; Luchicchi A.; Schenk G.J.; Joseph J.T.; Geurts J.J.G.; Stys P.K.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	The molecular composition of myelin membranes determines their structure and function. Even minute changes to the biochemical balance can have profound consequences for axonal conduction and the synchronicity of neural networks. Hypothesizing that the earliest indication of myelin injury involves changes in the composition and/or polarity of its constituent lipids, we developed a sensitive spectroscopic technique for defining the chemical polarity of myelin lipids in fixed frozen tissue sections from rodent and human. The method uses a simple staining procedure involving the lipophilic dye Nile Red, whose fluorescence spectrum varies according to the chemical polarity of the microenvironment into which the dye embeds. Nile Red spectroscopy identified histologically intact yet biochemically altered myelin in prelesioned tissues, including mouse white matter following subdemyelinating cuprizone intoxication, as well as normal-appearing white matter in multiple sclerosis brain. Nile Red spectroscopy offers a relatively simple yet highly sensitive technique for detecting subtle myelin changes. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cuprizone; Fluorescence spectroscopy; Lipids; Multiple sclerosis; Spectral confocal microscopy
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180528
作者单位	Department of Clinical Neurosciences, Hotchkiss Brain Institute, University of Calgary Cumming School of Medicine, Calgary, AB T2N 4N1, Canada; Department of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit, Amsterdam Neuroscience, Amsterdam, 1081 HZ, Netherlands; Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
推荐引用方式 GB/T 7714	Teo W.,Caprariello A.V.,Morgan M.L.,et al. Nile Red fluorescence spectroscopy reports early physicochemical changes in myelin with high sensitivity[J],2021,118(8).
APA	Teo W..,Caprariello A.V..,Morgan M.L..,Luchicchi A..,Schenk G.J..,...&Stys P.K..(2021).Nile Red fluorescence spectroscopy reports early physicochemical changes in myelin with high sensitivity.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Teo W.,et al."Nile Red fluorescence spectroscopy reports early physicochemical changes in myelin with high sensitivity".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).