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DOI	10.1073/pnas.2013315118
	Real-time measurements of aminoglycoside effects on protein synthesis in live cells
	Rivera J.A.; Larsson J.; Volkov I.L.; Seefeldt A.C.; Sanyal S.; Johansson M.
发表日期	2021
ISSN	00278424
卷号	118 期号:9
英文摘要	The spread of antibiotic resistance is turning many of the currently used antibiotics less effective against common infections. To address this public health challenge, it is critical to enhance our understanding of the mechanisms of action of these compounds. Aminoglycoside drugs bind the bacterial ribosome, and decades of results from in vitro biochemical and structural approaches suggest that these drugs disrupt protein synthesis by inhibiting the ribosome's translocation on the messenger RNA, as well as by inducing miscoding errors. So far, however, we have sparse information about the dynamic effects of these compounds on protein synthesis inside the cell. In the present study, we measured the effect of the aminoglycosides apramycin, gentamicin, and paromomycin on ongoing protein synthesis directly in live Escherichia coli cells by tracking the binding of dye-labeled transfer RNAs to ribosomes. Our results suggest that the drugs slow down translation elongation two- to fourfold in general, and the number of elongation cycles per initiation event seems to decrease to the same extent. Hence, our results imply that none of the drugs used in this study cause severe inhibition of translocation. © This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
英文关键词	Antibiotics; Single-molecule tracking; Superresolution microscopy; Translation; TRNA
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180502
作者单位	Department of Cell and Molecular Biology, Uppsala University, Uppsala, 752 36, Sweden
推荐引用方式 GB/T 7714	Rivera J.A.,Larsson J.,Volkov I.L.,et al. Real-time measurements of aminoglycoside effects on protein synthesis in live cells[J],2021,118(9).
APA	Rivera J.A.,Larsson J.,Volkov I.L.,Seefeldt A.C.,Sanyal S.,&Johansson M..(2021).Real-time measurements of aminoglycoside effects on protein synthesis in live cells.Proceedings of the National Academy of Sciences of the United States of America,118(9).
MLA	Rivera J.A.,et al."Real-time measurements of aminoglycoside effects on protein synthesis in live cells".Proceedings of the National Academy of Sciences of the United States of America 118.9(2021).