气候变化领域集成服务门户(CCPortal): Acetogenic bacteria utilize light-driven electrons as an energy source for autotrophic growth

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DOI	10.1073/pnas.2020552118
	Acetogenic bacteria utilize light-driven electrons as an energy source for autotrophic growth
	Jin S.; Jeon Y.; Jeon M.S.; Shin J.; Song Y.; Kang S.; Bae J.; Cho S.; Lee J.-K.; Kim D.R.; Cho B.-K.
发表日期	2021
ISSN	00278424
卷号	118 期号:9
英文摘要	Acetogenic bacteria use cellular redox energy to convert CO2 to acetate using the Wood-Ljungdahl (WL) pathway. Such redox energy can be derived from electrons generated from H2 as well as from inorganic materials, such as photoresponsive semiconductors. We have developed a nanoparticle-microbe hybrid system in which chemically synthesized cadmium sulfide nanoparticles (CdS-NPs) are displayed on the cell surface of the industrial acetogen Clostridium autoethanogenum. The hybrid system converts CO2 into acetate without the need for additional energy sources, such as H2, and uses only light-induced electrons from CdS-NPs. To elucidate the underlying mechanism by which C. autoethanogenum uses electrons generated from external energy sources to reduce CO2, we performed transcriptional analysis. Our results indicate that genes encoding the metal ion or flavin-binding proteins were highly up-regulated under CdS-driven autotrophic conditions along with the activation of genes associated with the WL pathway and energy conservation system. Furthermore, the addition of these cofactors increased the CO2 fixation rate under light-exposure conditions. Our results demonstrate the potential to improve the efficiency of artificial photosynthesis systems based on acetogenic bacteria integrated with photoresponsive nanoparticles. © This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Acetogenic bacteria; Artificial photosynthesis; Cadmium sulfide nanoparticle; Clostridium autoethanogenum; Extracellular electron transfer
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180491
作者单位	Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea; Innovative Biomaterials Research Center, KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141, South Korea; Department of Mechanical Engineering, Hanyang University, Seoul, 04763, South Korea; Department of Chemical Engineering, Konkuk University, Seoul, 05029, South Korea; Intelligent Synthetic Biology Center, Daejeon, 34141, South Korea
推荐引用方式 GB/T 7714	Jin S.,Jeon Y.,Jeon M.S.,et al. Acetogenic bacteria utilize light-driven electrons as an energy source for autotrophic growth[J],2021,118(9).
APA	Jin S..,Jeon Y..,Jeon M.S..,Shin J..,Song Y..,...&Cho B.-K..(2021).Acetogenic bacteria utilize light-driven electrons as an energy source for autotrophic growth.Proceedings of the National Academy of Sciences of the United States of America,118(9).
MLA	Jin S.,et al."Acetogenic bacteria utilize light-driven electrons as an energy source for autotrophic growth".Proceedings of the National Academy of Sciences of the United States of America 118.9(2021).