气候变化领域集成服务门户(CCPortal): Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses

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DOI	10.1073/pnas.2016754118
	Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses
	Fukaya R.; Maglione M.; Sigrist S.J.; Sakaba T.
发表日期	2021
ISSN	00278424
卷号	118 期号:9
英文摘要	The cyclic adenosine monophosphate (cAMP)-dependent potentiation of neurotransmitter release is important for higher brain functions such as learning and memory. To reveal the underlying mechanisms, we applied paired pre- and postsynaptic recordings from hippocampal mossy fiber-CA3 synapses. Ca2+ uncaging experiments did not reveal changes in the intracellular Ca2+ sensitivity for transmitter release by cAMP, but suggested an increase in the local Ca2+ concentration at the release site, which was much lower than that of other synapses before potentiation. Total internal reflection fluorescence (TIRF) microscopy indicated a clear increase in the local Ca2+ concentration at the release site within 5 to 10 min, suggesting that the increase in local Ca2+ is explained by the simple mechanism of rapid Ca2+ channel accumulation. Consistently, two-dimensional time-gated stimulated emission depletion microscopy (gSTED) microscopy showed an increase in the P/Q-type Ca2+ channel cluster size near the release sites. Taken together, this study suggests a potential mechanism for the cAMP-dependent increase in transmission at hippocampal mossy fiber-CA3 synapses, namely an accumulation of active zone Ca2+ channels. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Calcium channels; Hippocampal mossy fiber; Synapse; Synaptic plasticity; Synaptic transmission
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180489
作者单位	Graduate School of Brain Science, Doshisha University, Kyo-Tanabe, Kyoto, 6100394, Japan; Institute for Biology/Genetics, Freie Universität Berlin, Berlin, 14195, Germany; NeuroCure Cluster of Excellence, Charité Universitätsmedizin, Berlin, 10117, Germany
推荐引用方式 GB/T 7714	Fukaya R.,Maglione M.,Sigrist S.J.,et al. Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses[J],2021,118(9).
APA	Fukaya R.,Maglione M.,Sigrist S.J.,&Sakaba T..(2021).Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses.Proceedings of the National Academy of Sciences of the United States of America,118(9).
MLA	Fukaya R.,et al."Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses".Proceedings of the National Academy of Sciences of the United States of America 118.9(2021).