气候变化领域集成服务门户(CCPortal): A tandem activity-based sensing and labeling strategy enables imaging of transcellular hydrogen peroxide signaling

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DOI	10.1073/pnas.2018513118
	A tandem activity-based sensing and labeling strategy enables imaging of transcellular hydrogen peroxide signaling
	Iwashita H.; Castillo E.; Messina M.S.; Swanson R.A.; Chang C.J.
发表日期	2021
ISSN	00278424
卷号	118 期号:9
英文摘要	Reactive oxygen species (ROS) like hydrogen peroxide (H2O2) are transient species that have broad actions in signaling and stress, but spatioanatomical understanding of their biology remains insufficient. Here, we report a tandem activity-based sensing and labeling strategy for H2O2 imaging that enables capture and permanent recording of localized H2O2 fluxes. Peroxy Green-1 Fluoromethyl (PG1-FM) is a diffusible small-molecule probe that senses H2O2 by a boronate oxidation reaction to trigger dual release and covalent labeling of a fluorescent product, thus preserving spatial information on local H2O2 changes. This unique reagent enables visualization of transcellular redox signaling in a microglia-neuron coculture cell model, where selective activation of microglia for ROS production increases H2O2 in nearby neurons. In addition to identifying ROS-mediated cell-to-cell communication, this work provides a starting point for the design of chemical probes that can achieve high spatial fidelity by combining activity-based sensing and labeling strategies. © This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Activity-based sensing; Fluorescent hydrogen peroxide probe; NADPH oxidase; Oxidative stress; Redox signaling
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180478
作者单位	Department of Chemistry, University of California, Berkeley, CA 94720, United States; Department of Neurology, University of California, San Francisco and San Francisco Veterans Affairs Medical Center, San Francisco, CA 94143, United States; Neurology Service, San Francisco Veterans Affairs Health Care System, San Francisco, CA 94121, United States; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, United States; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Iwashita H.,Castillo E.,Messina M.S.,et al. A tandem activity-based sensing and labeling strategy enables imaging of transcellular hydrogen peroxide signaling[J],2021,118(9).
APA	Iwashita H.,Castillo E.,Messina M.S.,Swanson R.A.,&Chang C.J..(2021).A tandem activity-based sensing and labeling strategy enables imaging of transcellular hydrogen peroxide signaling.Proceedings of the National Academy of Sciences of the United States of America,118(9).
MLA	Iwashita H.,et al."A tandem activity-based sensing and labeling strategy enables imaging of transcellular hydrogen peroxide signaling".Proceedings of the National Academy of Sciences of the United States of America 118.9(2021).