气候变化领域集成服务门户(CCPortal): Cell cycle–dependent active stress drives epithelia remodeling

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DOI	10.1073/pnas.1917853118
	Cell cycle–dependent active stress drives epithelia remodeling
	Devany J.; Sussman D.M.; Yamamoto T.; Manning M.L.; Gardel M.L.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Epithelia have distinct cellular architectures which are established in development, reestablished after wounding, and maintained during tissue homeostasis despite cell turnover and mechanical perturbations. In turn, cell shape also controls tissue function as a regulator of cell differentiation, proliferation, and motility. Here, we investigate cell shape changes in a model epithelial monolayer. After the onset of confluence, cells continue to proliferate and change shape over time, eventually leading to a final architecture characterized by arrested motion and more regular cell shapes. Such monolayer remodeling is robust, with qualitatively similar evolution in cell shape and dynamics observed across disparate perturbations. Here, we quantify differences in monolayer remodeling guided by the active vertex model to identify underlying order parameters controlling epithelial architecture. When monolayers are formed atop an extracellular matrix with varied stiffness, we find the cell density at which motion arrests varies significantly, but the cell shape remains constant, consistent with the onset of tissue rigidity. In contrast, pharmacological perturbations can significantly alter the cell shape at which tissue dynamics are arrested, consistent with varied amounts of active stress within the tissue. Across all experimental conditions, the final cell shape is well correlated to the cell proliferation rate, and cell cycle inhibition immediately arrests cell motility. Finally, we demonstrate cell cycle variation in junctional tension as a source of active stress within the monolayer. Thus, the architecture and mechanics of epithelial tissue can arise from an interplay between cell mechanics and stresses arising from cell cycle dynamics. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell cycle; Cell mechanics; Epithelial tissue; Vertex model
语种	英语
scopus关键词	article; cell density; cell motility; cell proliferation; cell shape; controlled study; epithelium; extracellular matrix; motion; physiological stress; quantitative analysis; rigidity; tension
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180402
作者单位	Department of Physics, Institute for Biophysical Dynamics, James Franck Institute, University of Chicago, Chicago, IL 60637, United States; Department of Physics, BioInspired Institute, Syracuse University, Syracuse, NY 13244, United States; Department of Physics, Emory University, Atlanta, GA 30322, United States; Nonequilibrium Physics of Living Matter RIKEN Hakubi Research Team, RIKEN Center for Biosystems Dynamics Research, Kobe, 650-0047, Japan; Pritzker School of Molecular Engineering, University of Chicago, Chicago, IL 60637, United States
推荐引用方式 GB/T 7714	Devany J.,Sussman D.M.,Yamamoto T.,等. Cell cycle–dependent active stress drives epithelia remodeling[J],2021,118(10).
APA	Devany J.,Sussman D.M.,Yamamoto T.,Manning M.L.,&Gardel M.L..(2021).Cell cycle–dependent active stress drives epithelia remodeling.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Devany J.,et al."Cell cycle–dependent active stress drives epithelia remodeling".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).