气候变化领域集成服务门户(CCPortal): Adaptive evolution of hybrid bacteria by horizontal gene transfer

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DOI	10.1073/pnas.2007873118
	Adaptive evolution of hybrid bacteria by horizontal gene transfer
	Power J.J.; Pinheiro F.; Pompei S.; Kovacova V.; Yüksel M.; Rathmann I.; Förster M.; Lässig M.; Maier B.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Horizontal gene transfer (HGT) is an important factor in bacterial evolution that can act across species boundaries. Yet, we know little about rate and genomic targets of cross-lineage gene transfer and about its effects on the recipient organism’s physiology and fitness. Here, we address these questions in a parallel evolution experiment with two Bacillus subtilis lineages of 7% sequence divergence. We observe rapid evolution of hybrid organisms: gene transfer swaps ∼12% of the core genome in just 200 generations, and 60% of core genes are replaced in at least one population. By genomics, transcriptomics, fitness assays, and statistical modeling, we show that transfer generates adaptive evolution and functional alterations in hybrids. Specifically, our experiments reveal a strong, repeatable fitness increase of evolved populations in the stationary growth phase. By genomic analysis of the transfer statistics across replicate populations, we infer that selection on HGT has a broad genetic basis: 40% of the observed transfers are adaptive. At the level of functional gene networks, we find signatures of negative, positive, and epistatic selection, consistent with hybrid incompatibilities and adaptive evolution of network functions. Our results suggest that gene transfer navigates a complex cross-lineage fitness landscape, bridging epistatic barriers along multiple high-fitness paths. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Experimental evolution; Fitness landscape; Horizontal gene transfer
语种	英语
scopus关键词	article; Bacillus subtilis; genomics; growth curve; horizontal gene transfer; nonhuman; transcriptomics
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180393
作者单位	Institute for Biological Physics, University of Cologne, Köln, 50937, Germany; Center for Molecular Medicine Cologne, University of Cologne, Köln, 50923, Germany
推荐引用方式 GB/T 7714	Power J.J.,Pinheiro F.,Pompei S.,et al. Adaptive evolution of hybrid bacteria by horizontal gene transfer[J],2021,118(10).
APA	Power J.J..,Pinheiro F..,Pompei S..,Kovacova V..,Yüksel M..,...&Maier B..(2021).Adaptive evolution of hybrid bacteria by horizontal gene transfer.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Power J.J.,et al."Adaptive evolution of hybrid bacteria by horizontal gene transfer".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).