气候变化领域集成服务门户(CCPortal): Spine dynamics of PSD-95-deficient neurons in the visual cortex link silent synapses to structural cortical plasticity

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DOI	10.1073/pnas.2022701118
	Spine dynamics of PSD-95-deficient neurons in the visual cortex link silent synapses to structural cortical plasticity
	Yusifov R.; Tippmann A.; Staiger J.F.; Schlüter O.M.; Löwel S.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Critical periods (CPs) are time windows of heightened brain plasticity during which experience refines synaptic connections to achieve mature functionality. At glutamatergic synapses on dendritic spines of principal cortical neurons, the maturation is largely governed by postsynaptic density protein-95 (PSD-95)-dependent synaptic incorporation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors into nascent AMPA-receptor silent synapses. Consequently, in mouse primary visual cortex (V1), impaired silent synapse maturation in PSD-95-deficient neurons prevents the closure of the CP for juvenile ocular dominance plasticity (jODP). A structural hallmark of jODP is increased spine elimination, induced by brief monocular deprivation (MD). However, it is unknown whether impaired silent synapse maturation facilitates spine elimination and also preserves juvenile structural plasticity. Using two-photon microscopy, we assessed spine dynamics in apical dendrites of layer 2/3 pyramidal neurons (PNs) in binocular V1 during ODP in awake adult mice. Under basal conditions, spine formation and elimination ratios were similar between PSD-95 knockout (KO) and wild-type (WT) mice. However, a brief MD affected spine dynamics only in KO mice, where MD doubled spine elimination, primarily affecting newly formed spines, and caused a net reduction in spine density similar to what has been observed during jODP in WT mice. A similar increase in spine elimination after MD occurred if PSD-95 was knocked down in single PNs of layer 2/3. Thus, structural plasticity is dictated cell autonomously by PSD-95 in vivo in awake mice. Loss of PSD-95 preserves hallmark features of spine dynamics in jODP into adulthood, revealing a functional link of PSD-95 for experience-dependent synapse maturation and stabilization during CPs. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Awake; Plasticity; Silent synapses; Spine dynamics; Visual cortex
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180388
作者单位	Department of Systems Neuroscience, Johann Friedrich Blumenbach Institut für Zoologie und Anthropologie, Universität Göttingen, Göttingen, D-37075, Germany; Collaborative Research Center 889, Universität Göttingen, Göttingen, D-37075, Germany; Campus Institute for Dynamics of Biological Networks, Universität Göttingen, Göttingen, D-37075, Germany; Institute for Neuroanatomy, University Medical Center, Universität Göttingen, Göttingen, D-37075, Germany; Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA 15260, United States; Department of Psychiatry and Psychotherapy, University Medical Center Göttingen, Universität Göttingen, Göttingen, D-37075, Germany
推荐引用方式 GB/T 7714	Yusifov R.,Tippmann A.,Staiger J.F.,et al. Spine dynamics of PSD-95-deficient neurons in the visual cortex link silent synapses to structural cortical plasticity[J],2021,118(10).
APA	Yusifov R.,Tippmann A.,Staiger J.F.,Schlüter O.M.,&Löwel S..(2021).Spine dynamics of PSD-95-deficient neurons in the visual cortex link silent synapses to structural cortical plasticity.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Yusifov R.,et al."Spine dynamics of PSD-95-deficient neurons in the visual cortex link silent synapses to structural cortical plasticity".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).