气候变化领域集成服务门户(CCPortal): Lipid droplets in mammalian eggs are utilized during embryonic diapause

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DOI	10.1073/pnas.2018362118
	Lipid droplets in mammalian eggs are utilized during embryonic diapause
	Arena R.; Bisogno S.; Gasior Ł.; Rudnicka J.; Bernhardt L.; Haaf T.; Zacchini F.; Bochenek M.; Fic K.; Bik E.; Baranska M.; Bodzon-Ku łakowska A.; Suder P.; Depciuch J.; Gurgul A.; Polanski Z.; Ptak G.E.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Embryonic diapause (ED) is a temporary arrest of an embryo at the blastocyst stage when it waits for the uterine receptivity signal to implant. ED used by over 100 species may also occur in normally “nondiapausing” mammals when the uterine receptivity signal is blocked or delayed. A large number of lipid droplets (LDs) are stored throughout the preimplantation embryo development, but the amount of lipids varies greatly across different mammalian species. Yet, the role of LDs in the mammalian egg and embryo remains unknown. Here, using a mouse model, we provide evidence that LDs play a crucial role in maintaining ED. By mechanical removal of LDs from zygotes, we demonstrated that delipidated embryos are unable to survive during ED. LDs are not essential for normal prompt implantation, without ED. We further demonstrated that with the progression of ED, the amount of intracellular lipid reduces, and composition changes. This decrease in lipid is caused by a switch from carbohydrate metabolism to lipid catabolism in diapausing blastocysts, which also exhibit increased release of exosomes reflecting elevated embryonic signaling to the mother. We have also shown that presence of LDs in the oocytes of various mammals positively corelates with their species-specific length of diapause. Our results reveal the functional role of LDs in embryonic development. These results can help to develop diagnostic techniques and treatment of recurrent implantation failure and will likely ignite further studies in developmental biology and reproductive medicine fields. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Blastocyst; Embryonic diapause; Lipid droplets
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180374
作者单位	Małopolska Centre of Biotechnology, Jagiellonian University, Krakow, 30-387, Poland; Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrze¸ biec, 05-552, Poland; Institute of Human Genetics, Julius Maximilian University, Würzburg, 97074, Germany; Percuros BV, Leiden, 2333, Netherlands; Faculty of Chemistry, Jagiellonian University, Krakow, 30-387, Poland; Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, Krakow, 30-348, Poland; Department of Biochemistry and Neurobiology, Akademia Górniczo-Hutnicza University of Science and Technology, Krakow, 30-059, Poland; Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, 31-342, Poland; University Centre of Veterinary Medicine, University of Agriculture, Krakow, 30-059, Poland; Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, 30-387, Poland; Department of Biosciences, University of Teramo, Teramo, 64100, Italy
推荐引用方式 GB/T 7714	Arena R.,Bisogno S.,Gasior Ł.,et al. Lipid droplets in mammalian eggs are utilized during embryonic diapause[J],2021,118(10).
APA	Arena R..,Bisogno S..,Gasior Ł..,Rudnicka J..,Bernhardt L..,...&Ptak G.E..(2021).Lipid droplets in mammalian eggs are utilized during embryonic diapause.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Arena R.,et al."Lipid droplets in mammalian eggs are utilized during embryonic diapause".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).