气候变化领域集成服务门户(CCPortal): Transcriptional heterogeneity and tightly regulated changes in gene expression during Plasmodium berghei sporozoite development

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DOI	10.1073/pnas.2023438118
	Transcriptional heterogeneity and tightly regulated changes in gene expression during Plasmodium berghei sporozoite development
	Bogale H.N.; Pascini T.V.; Kanatani S.; Sá J.M.; Wellems T.E.; Sinnis P.; Vega-Rodríguez J.; Serre D.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Despite the critical role of Plasmodium sporozoites in malaria transmission, we still know little about the mechanisms underlying their development in mosquitoes. Here, we use single-cell RNA sequencing to characterize the gene expression profiles of 16,038 Plasmodium berghei sporozoites isolated throughout their development from midgut oocysts to salivary glands, and from forced salivation experiments. Our results reveal a succession of tightly regulated changes in gene expression occurring during the maturation of sporozoites and highlight candidate genes that could play important roles in oocyst egress, sporozoite motility, and the mechanisms underlying the invasion of mosquito salivary glands and mammalian hepatocytes. In addition, the single-cell data reveal extensive transcriptional heterogeneity among parasites isolated from the same anatomical site, suggesting that Plasmodium development in mosquitoes is asynchronous and regulated by intrinsic as well as environmental factors. Finally, our analyses show a decrease in transcriptional activity preceding the translational repression observed in mature sporozoites and associated with their quiescent state in salivary glands, followed by a rapid reactivation of the transcriptional machinery immediately upon salivation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Gene expression; Malaria; Single-cell analysis
语种	英语
scopus关键词	article; environmental factor; gene expression profiling; liver cell; mammal; midgut; mosquito; nonhuman; oocyst; Plasmodium; Plasmodium berghei; salivation; single cell analysis; single cell RNA seq; sporozoite; transcription initiation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180369
作者单位	Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, United States; Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States; Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, United States; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, United States
推荐引用方式 GB/T 7714	Bogale H.N.,Pascini T.V.,Kanatani S.,et al. Transcriptional heterogeneity and tightly regulated changes in gene expression during Plasmodium berghei sporozoite development[J],2021,118(10).
APA	Bogale H.N..,Pascini T.V..,Kanatani S..,Sá J.M..,Wellems T.E..,...&Serre D..(2021).Transcriptional heterogeneity and tightly regulated changes in gene expression during Plasmodium berghei sporozoite development.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Bogale H.N.,et al."Transcriptional heterogeneity and tightly regulated changes in gene expression during Plasmodium berghei sporozoite development".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).