气候变化领域集成服务门户(CCPortal): Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway

CCPortal

DOI	10.1073/pnas.2020429118
	Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway
	Hurtig J.E.; Steiger M.A.; Nagarajan V.K.; Li T.; Chao T.-C.; Tsai K.-L.; Van Hoof A.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Eukaryotes share a conserved messenger RNA (mRNA) decay pathway in which bulk mRNA is degraded by exoribonucleases. In addition, it has become clear that more specialized mRNA decay pathways are initiated by endonucleolytic cleavage at particular sites. The transfer RNA (tRNA) splicing endonuclease (TSEN) has been studied for its ability to remove introns from pre-tRNAs. More recently it has been shown that single amino acid mutations in TSEN cause pontocerebellar hypoplasia. Other recent studies indicate that TSEN has other functions, but the nature of these functions has remained obscure. Here we show that yeast TSEN cleaves a specific subset of mRNAs that encode mitochondrial proteins, and that the cleavage sites are in part determined by their sequence. This provides an explanation for the counterintuitive mitochondrial localization of yeast TSEN. To identify these mRNA target sites, we developed a “comPARE” (comparative parallel analysis of RNA ends) bioinformatic approach that should be easily implemented and widely applicable to the study of endoribonucleases. The similarity of tRNA endonuclease-initiated decay to regulated IRE1-dependent decay of mRNA suggests that mRNA specificity by colocalization may be an important determinant for the degradation of localized mRNAs in a variety of eukaryotic cells. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Endonuclease; MRNA degradation; Trna splicing
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180359
作者单位	Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, TX 77030, United States; Department of Chemistry and Biochemistry, University of St. Thomas, Houston, TX 77006, United States; Delaware Biotechnology Institute, University of Delaware, Newark, DE 19713, United States; Biochemistry and Molecular Biology, University of Texas Health Science Center, Houston, TX 77030, United States
推荐引用方式 GB/T 7714	Hurtig J.E.,Steiger M.A.,Nagarajan V.K.,et al. Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway[J],2021,118(10).
APA	Hurtig J.E..,Steiger M.A..,Nagarajan V.K..,Li T..,Chao T.-C..,...&Van Hoof A..(2021).Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Hurtig J.E.,et al."Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).