气候变化领域集成服务门户(CCPortal): Cas9-expressing chickens and pigs as resources for genome editing in livestock

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DOI	10.1073/pnas.2022562118
	Cas9-expressing chickens and pigs as resources for genome editing in livestock
	Rieblinger B.; Sid H.; Duda D.; Bozoglu T.; Klinger R.; Schlickenrieder A.; Lengyel K.; Flisikowski K.; Flisikowska T.; Simm N.; Grodziecki A.; Perleberg C.; Bähr A.; Carrier L.; Kurome M.; Zakhartchenko V.; Kessler B.; Wolf E.; Kettler L.; Luksch H.; Hagag I.T.; Wise D.; Kaufman J.; Kaufer B.B.; Kupatt C.; Schnieke A.; Schusser B.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Genetically modified animals continue to provide important insights into the molecular basis of health and disease. Research has focused mostly on genetically modified mice, although other species like pigs resemble the human physiology more closely. In addition, cross-species comparisons with phylogenetically distant species such as chickens provide powerful insights into fundamental biological and biomedical processes. One of the most versatile genetic methods applicable across species is CRISPR-Cas9. Here, we report the generation of transgenic chickens and pigs that constitutively express Cas9 in all organs. These animals are healthy and fertile. Functionality of Cas9 was confirmed in both species for a number of different target genes, for a variety of cell types and in vivo by targeted gene disruption in lymphocytes and the developing brain, and by precise excision of a 12.7-kb DNA fragment in the heart. The Cas9 transgenic animals will provide a powerful resource for in vivo genome editing for both agricultural and translational biomedical research, and will facilitate reverse genetics as well as cross-species comparisons. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cas9 transgenic chicken; Cas9 transgenic pig; CRISPR-Cas9; In vivo genome editing
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180340
作者单位	Livestock Biotechnology, Department of Molecular Life Sciences, School of Life Sciences Weihenstephan, Technical University Munich, Freising, 85354, Germany; Reproductive Biotechnology, Department of Molecular Life Sciences, School of Life Sciences Weihenstephan, Technical University Munich, Freising, 85354, Germany; Clinic and Polyclinic for Internal Medicine I, Klinikum rechts der Isar, Technical University Munich, Munich, 81675, Germany; Munich Heart Alliance, German Center for Cardiovascular Research, Munich, 81675, Germany; Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, 20246, Germany; Institute of Experimental Pharmacology and Toxicology, German Centre for Cardiovascular Research, Hamburg, 20246, Germany; Gene Center, Ludwig-Maximilians-Universität München, Munich, 81377, Germany; Molecular Animal Breeding and Biotechnology, Department of Veterinary Sciences, Ludwig-Maximilians-Universität München, München, 81377, Germany; Zoology, Depart...
推荐引用方式 GB/T 7714	Rieblinger B.,Sid H.,Duda D.,et al. Cas9-expressing chickens and pigs as resources for genome editing in livestock[J],2021,118(10).
APA	Rieblinger B..,Sid H..,Duda D..,Bozoglu T..,Klinger R..,...&Schusser B..(2021).Cas9-expressing chickens and pigs as resources for genome editing in livestock.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Rieblinger B.,et al."Cas9-expressing chickens and pigs as resources for genome editing in livestock".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).