气候变化领域集成服务门户(CCPortal): Negative regulation of plastidial isoprenoid pathway by herbivore-induced β-cyclocitral in Arabidopsis thaliana

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DOI	10.1073/pnas.2008747118
	Negative regulation of plastidial isoprenoid pathway by herbivore-induced β-cyclocitral in Arabidopsis thaliana
	Mitra S.; Estrada-Tejedor R.; Volke D.C.; Phillips M.A.; Gershenzon J.; Wright L.P.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Insect damage to plants is known to up-regulate defense and down-regulate growth processes. While there are frequent reports about up-regulation of defense signaling and production of defense metabolites in response to herbivory, much less is understood about the mechanisms by which growth and carbon assimilation are down-regulated. Here we demonstrate that insect herbivory down-regulates the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway in Arabidopsis (Arabidopsis thaliana), a pathway making primarily metabolites for use in photosynthesis. Simulated feeding by the generalist herbivore Spodoptera littoralis suppressed flux through the MEP pathway and decreased steady-state levels of the intermediate 1-deoxy-D-xylulose 5-phosphate (DXP). Simulated herbivory also increased reactive oxygen species content which caused the conversion of β-carotene to β-cyclocitral (βCC). This volatile oxidation product affected the MEP pathway by directly inhibiting DXP synthase (DXS), the rate-controlling enzyme of the MEP pathway in Arabidopsis and inducing plant resistance against S. littoralis. βCC inhibited both DXS transcript accumulation and DXS activity. Molecular models suggested that βCC binds to DXS at the binding site for the thymine pyrophosphate cofactor and blocks catalysis, which was confirmed by direct assays of βCC with the purified DXS protein in vitro. Another intermediate of the MEP pathway, 2-C-methyl-D-erythritol-2, 4-cyclodiphosphate, which is known to stimulate salicylate defense signaling, showed greater accumulation and enhanced export out of the plastid in response to simulated herbivory. Together, our work implicates βCC as a signal of herbivore damage in Arabidopsis that increases defense and decreases flux through the MEP pathway, a pathway involved in growth and carbon assimilation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Apocarotenoids; Isoprenoid; Mechanical damage; Methylerythritol-4phosphate pathway; Simulated herbivory
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180314
作者单位	Department of Biochemistry, Max Planck Institute for Chemical Ecology, Jena, 07745, Germany; Department of Botany, Savitribai Phule Pune University, Pune, 411007, India; Pharmaceutical Chemistry Group, IQS School of Engineering, Universitat Ramon Llull, Barcelona, 08017, Spain; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark; Department of Biology, University of Toronto Mississauga, Mississauga, ON L5L 1C6, Canada
推荐引用方式 GB/T 7714	Mitra S.,Estrada-Tejedor R.,Volke D.C.,et al. Negative regulation of plastidial isoprenoid pathway by herbivore-induced β-cyclocitral in Arabidopsis thaliana[J],2021,118(10).
APA	Mitra S.,Estrada-Tejedor R.,Volke D.C.,Phillips M.A.,Gershenzon J.,&Wright L.P..(2021).Negative regulation of plastidial isoprenoid pathway by herbivore-induced β-cyclocitral in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Mitra S.,et al."Negative regulation of plastidial isoprenoid pathway by herbivore-induced β-cyclocitral in Arabidopsis thaliana".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).