气候变化领域集成服务门户(CCPortal): A synthetic 5,3-cross-link in the cell wall of rod-shaped Gram-positive bacteria View ORCID Profile

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DOI	10.1073/pnas.2100137118
	A synthetic 5,3-cross-link in the cell wall of rod-shaped Gram-positive bacteria View ORCID Profile
	Dik D.A.; Zhang N.; Sturgell E.J.; Sanchez B.B.; Chen J.S.; Webb B.; Vanderpool K.G.; Schultz P.G.
发表日期	2021
ISSN	00278424
卷号	118 期号:11
英文摘要	Gram-positive bacteria assemble a multilayered cell wall that provides tensile strength to the cell. The cell wall is composed of glycan strands cross-linked by nonribosomally synthesized peptide stems. Herein, we modify the peptide stems of the Grampositive bacterium Bacillus subtilis with noncanonical electrophilic D-amino acids, which when in proximity to adjacent stem peptides form novel covalent 5,3-cross-links. Approximately 20% of canonical cell-wall cross-links can be replaced with synthetic cross-links. While a low level of synthetic cross-link formation does not affect B. subtilis growth and phenotype, at higher levels cell growth is perturbed and bacteria elongate. A comparison of the accumulation of synthetic cross-links over time in Gram-negative and Grampositive bacteria highlights key differences between them. The ability to perturb cell-wall architecture with synthetic building blocks provides a novel approach to studying the adaptability, elasticity, and porosity of bacterial cell walls. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacteria; Cell wall; Synthetic cross-links; Transpeptidases
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180280
作者单位	Department of Chemistry, Scripps Research, San Diego, CA 92037, United States; Automated Synthesis Facility, Scripps Research, San Diego, CA 92037, United States; Center for Metabolomics and Mass Spectrometry, Scripps Research, San Diego, CA 92037, United States; Core Microscopy Facility, Scripps Research, San Diego, CA 92037, United States
推荐引用方式 GB/T 7714	Dik D.A.,Zhang N.,Sturgell E.J.,et al. A synthetic 5,3-cross-link in the cell wall of rod-shaped Gram-positive bacteria View ORCID Profile[J],2021,118(11).
APA	Dik D.A..,Zhang N..,Sturgell E.J..,Sanchez B.B..,Chen J.S..,...&Schultz P.G..(2021).A synthetic 5,3-cross-link in the cell wall of rod-shaped Gram-positive bacteria View ORCID Profile.Proceedings of the National Academy of Sciences of the United States of America,118(11).
MLA	Dik D.A.,et al."A synthetic 5,3-cross-link in the cell wall of rod-shaped Gram-positive bacteria View ORCID Profile".Proceedings of the National Academy of Sciences of the United States of America 118.11(2021).