气候变化领域集成服务门户(CCPortal): A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity

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DOI	10.1073/pnas.2016287118
	A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity
	Zdravković A.; Daley J.M.; Dutta A.; Niwa T.; Murayama Y.; Kanamaru S.; Ito K.; Maki T.; Argunhan B.; Takahashi M.; Tsubouchi H.; Sung P.; Iwasaki H.
发表日期	2021
ISSN	00278424
卷号	118 期号:11
英文摘要	The Mre11-Rad50-Nbs1 complex (MRN) is important for repairing DNA double-strand breaks (DSBs) by homologous recombination (HR). The endonuclease activity of MRN is critical for resecting 5′- ended DNA strands at DSB ends, producing 3′-ended single-strand DNA, a prerequisite for HR. This endonuclease activity is stimulated by Ctp1, the Schizosaccharomyces pombe homolog of human CtIP. Here, with purified proteins, we show that Ctp1 phosphorylation stimulatesMRN endonuclease activity by inducing the association of Ctp1 with Nbs1. The highly conserved extreme C terminus of Ctp1 is indispensable for MRN activation. Importantly, a polypeptide composed of the conserved 15 amino acids at the C terminus of Ctp1 (CT15) is sufficient to stimulate Mre11 endonuclease activity. Furthermore, the CT15 equivalent from CtIP can stimulate human MRE11 endonuclease activity, arguing for the generality of this stimulatory mechanism. Thus, we propose that Nbs1-mediated recruitment of CT15 plays a pivotal role in the activation of the Mre11 endonuclease by Ctp1/CtIP. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Ctp1/CtIP; Double-strand break repair; Fission yeast; Homologous recombination; Mre11-Rad50-Nbs1
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180241
作者单位	School and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa, 226-8503, Japan; Institute of Innovative Research, Tokyo Institute of Technology, Kanagawa, 226-8503, Japan; Department of Biochemistry and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78229, United States; Center for Frontier Research, National Institute of Genetics, Shizuoka, 411-8540, Japan
推荐引用方式 GB/T 7714	Zdravković A.,Daley J.M.,Dutta A.,et al. A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity[J],2021,118(11).
APA	Zdravković A..,Daley J.M..,Dutta A..,Niwa T..,Murayama Y..,...&Iwasaki H..(2021).A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity.Proceedings of the National Academy of Sciences of the United States of America,118(11).
MLA	Zdravković A.,et al."A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity".Proceedings of the National Academy of Sciences of the United States of America 118.11(2021).