气候变化领域集成服务门户(CCPortal): Quantification of Brucella abortus population structure in a natural host

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DOI	10.1073/pnas.2023500118
	Quantification of Brucella abortus population structure in a natural host
	Fiebig A.; Vrentas C.E.; Le T.; Huebner M.; Boggiatto P.M.; Olsen S.C.; Crosson S.
发表日期	2021
ISSN	00278424
卷号	118 期号:11
英文摘要	Cattle are natural hosts of the intracellular pathogen Brucella abortus, which inflicts a significant burden on the health and reproduction of these important livestock. The primary routes of infection in field settings have been described, but it is not known how the bovine host shapes the structure of B. abortus populations during infection. We utilized a library of uniquely barcoded B. abortus strains to temporally and spatially quantify population structure during colonization of cattle through a natural route of infection. Introducing 108bacteria from this barcoded library to the conjunctival mucosa resulted in expected levels of local lymph node colonization at a 1-wk time point. We leveraged variance in strain abundance in the library to demonstrate that only 1 in 10,000 brucellae introduced at the site of infection reached a parotid lymph node. Thus, cattle restrict the overwhelming majority of B. abortus introduced via the ocular conjunctiva at this dose. Individual strains were spatially restricted within the host tissue, and the total B. abortus census was dominated by a small number of distinct strains in each lymph node. These results define a bottleneck that B. abortus must traverse to colonize local lymph nodes from the conjunctival mucosa. The data further support a model in which a small number of spatially isolated granulomas founded by unique strains are present at 1 wk postinfection. These experiments demonstrate the power of barcoded transposon tools to quantify infection bottlenecks and to define pathogen population structure in host tissues. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bottleneck; Bovine; Brucellosis; Infectious dose; TnSeq
语种	英语
scopus关键词	Article; bacterial colonization; bacterial load; bacterial strain; bovine; Brucella abortus; brucellosis; conjunctiva; conjunctival mucosa; DNA barcoding; gene amplification; gene sequence; host bacterium interaction; lymph node; microbial population dynamics; natural host; nonhuman; parotid gland; population structure; priority journal; quantitative analysis; transposon
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180229
作者单位	Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States; Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010, United States; Department of Statistics and Probability, Michigan State University, East Lansing, MI 48824, United States
推荐引用方式 GB/T 7714	Fiebig A.,Vrentas C.E.,Le T.,et al. Quantification of Brucella abortus population structure in a natural host[J],2021,118(11).
APA	Fiebig A..,Vrentas C.E..,Le T..,Huebner M..,Boggiatto P.M..,...&Crosson S..(2021).Quantification of Brucella abortus population structure in a natural host.Proceedings of the National Academy of Sciences of the United States of America,118(11).
MLA	Fiebig A.,et al."Quantification of Brucella abortus population structure in a natural host".Proceedings of the National Academy of Sciences of the United States of America 118.11(2021).