气候变化领域集成服务门户(CCPortal): Zinc finger protein E4F1 cooperates with PARP-1 and BRG1 to promote DNA double-strand break repair

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DOI	10.1073/pnas.2019408118
	Zinc finger protein E4F1 cooperates with PARP-1 and BRG1 to promote DNA double-strand break repair
	Moison C.; Chagraoui J.; Caron M.-C.; Gagné J.-P.; Coulombe Y.; Poirier G.G.; Masson J.-Y.; Sauvageau G.
发表日期	2021
ISSN	00278424
卷号	118 期号:11
英文摘要	Zinc finger (ZnF) proteins represent one of the largest families of human proteins, although most remain uncharacterized. Given that numerous ZnF proteins are able to interact with DNA and poly(ADP ribose), there is growing interest in understanding their mechanism of action in the maintenance of genome integrity. We now report that the ZnF protein E4F transcription factor 1 (E4F1) is an actor in DNA repair. Indeed, E4F1 is rapidly recruited, in a poly(ADP ribose) polymerase (PARP)-dependent manner, to DNA breaks and promotes ATR/CHK1 signaling, DNA-end resection, and subsequent homologous recombination. Moreover, we identify E4F1 as a regulator of the ATP-dependent chromatin remodeling SWI/SNF complex in DNA repair. E4F1 binds to the catalytic subunit BRG1/SMARCA4 and together with PARP-1 mediates its recruitment to DNA lesions. We also report that a proportion of human breast cancers show amplification and overexpression of E4F1 or BRG1 that are mutually exclusive with BRCA1/2 alterations. Together, these results reveal a function of E4F1 in the DNA damage response that orchestrates proper signaling and repair of double-strand breaks and document a molecular mechanism for its essential role in maintaining genome integrity and cell survival. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Breast cancer; BRG1; Double-strand break; E4F1; PARP-1
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180222
作者单位	Molecular Genetics of Stem Cells Laboratory, Institute for Research in Immunology and Cancer, University of Montreal, Montreal, QC H3T 1J4, Canada; Genome Stability Laboratory, Oncology Division, CHU de Québec Research Center, Quebec City, QC G1R 3S3, Canada; Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University Cancer Research Center, Quebec City, QC G1V 0A6, Canada; Oncology Division, CHU de Québec Research Center, Quebec City, QC G1V 4G2, Canada; Department of Medicine, Faculty of Medicine, University of Montreal, Montreal, QC H3T 1J4, Canada; Division of Hematology, Maisonneuve-Rosemont Hospital, Montreal, QC H1T 2M4, Canada
推荐引用方式 GB/T 7714	Moison C.,Chagraoui J.,Caron M.-C.,et al. Zinc finger protein E4F1 cooperates with PARP-1 and BRG1 to promote DNA double-strand break repair[J],2021,118(11).
APA	Moison C..,Chagraoui J..,Caron M.-C..,Gagné J.-P..,Coulombe Y..,...&Sauvageau G..(2021).Zinc finger protein E4F1 cooperates with PARP-1 and BRG1 to promote DNA double-strand break repair.Proceedings of the National Academy of Sciences of the United States of America,118(11).
MLA	Moison C.,et al."Zinc finger protein E4F1 cooperates with PARP-1 and BRG1 to promote DNA double-strand break repair".Proceedings of the National Academy of Sciences of the United States of America 118.11(2021).