气候变化领域集成服务门户(CCPortal): A co-opted steroid synthesis gene, maintained in sorghum but not maize, is associated with a divergence in leaf wax chemistry

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DOI	10.1073/pnas.2022982118
	A co-opted steroid synthesis gene, maintained in sorghum but not maize, is associated with a divergence in leaf wax chemistry
	Busta L.; Schmitz E.; Kosma D.K.; Schnable J.C.; Cahoon E.B.
发表日期	2021
ISSN	00278424
卷号	118 期号:12
英文摘要	Virtually all land plants are coated in a cuticle, a waxy polyester that prevents nonstomatal water loss and is important for heat and drought tolerance. Here, we describe a likely genetic basis for a divergence in cuticular wax chemistry between Sorghum bicolor, a drought tolerant crop widely cultivated in hot climates, and its close relative Zea mays (maize). Combining chemical analyses, heterologous expression, and comparative genomics, we reveal that: 1) sorghum and maize leaf waxes are similar at the juvenile stage but, after the juvenile-to-adult transition, sorghum leaf waxes are rich in triterpenoids that are absent from maize; 2) biosynthesis of the majority of sorghum leaf triterpenoids is mediated by a gene that maize and sorghum both inherited from a common ancestor but that is only functionally maintained in sorghum; and 3) sorghum leaf triterpenoids accumulate in a spatial pattern that was previously shown to strengthen the cuticle and decrease water loss at high temperatures. These findings uncover the possibility for resurrection of a cuticular triterpenoid-synthesizing gene in maize that could create a more heat-tolerant water barrier on the plant’s leaf surfaces. They also provide a fundamental understanding of sorghum leaf waxes that will inform efforts to divert surface carbon to intracellular storage for bioenergy and bioproduct innovations. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cuticular wax \| drought tolerance \| triterpenoids \| juvenile-to-adult transition \| sorghum bicolor
语种	英语
scopus关键词	carbon; lanosterol synthase; triterpenoid; adult; Article; bioaccumulation; bioenergy; biosynthesis; chemical analysis; chemical structure; climate; codon; controlled study; developmental stage; drought tolerance; gene expression; gene synthesis; genetic variability; genomics; heat tolerance; high temperature; maize; mass fragmentography; nonhuman; plant leaf; plant tissue; priority journal; protein expression; RNA sequencing; sorghum; species cultivation; steroidogenesis; thin layer chromatography; water loss
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180193
作者单位	Department of Biochemistry, University of Nebraska–Lincoln, Lincoln, NE 68588, United States; Center for Plant Science Innovation, University of Nebraska–Lincoln, Lincoln, NE 68588, United States; Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, United States; Department of Agronomy and Horticulture, University of Nebraska–Lincoln, Lincoln, NE 68583, United States
推荐引用方式 GB/T 7714	Busta L.,Schmitz E.,Kosma D.K.,et al. A co-opted steroid synthesis gene, maintained in sorghum but not maize, is associated with a divergence in leaf wax chemistry[J],2021,118(12).
APA	Busta L.,Schmitz E.,Kosma D.K.,Schnable J.C.,&Cahoon E.B..(2021).A co-opted steroid synthesis gene, maintained in sorghum but not maize, is associated with a divergence in leaf wax chemistry.Proceedings of the National Academy of Sciences of the United States of America,118(12).
MLA	Busta L.,et al."A co-opted steroid synthesis gene, maintained in sorghum but not maize, is associated with a divergence in leaf wax chemistry".Proceedings of the National Academy of Sciences of the United States of America 118.12(2021).