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DOI	10.1073/pnas.2019996118
	Mono- and bilayer smectic liquid crystal ordering in dense solutions of “gapped” DNA duplexes
	Gyawali P.; Saha R.; Smith G.P.; Salamonczyk M.; Kharel P.; Basu S.; Li R.; Fukuto M.; Gleeson J.T.; Clark N.A.; Jákli A.; Balci H.; Sprunt S.
发表日期	2021
ISSN	00278424
卷号	118 期号:12
英文摘要	Although its mesomorphic properties have been studied for many years, only recently has the molecule of life begun to reveal the true range of its rich liquid crystalline behavior. End-to-end interactions between concentrated, ultrashort DNA duplexes—driving the self-assembly of aggregates that organize into liquid crystal phases—and the incorporation of flexible single-stranded “gaps” in otherwise fully paired duplexes—producing clear evidence of an elementary lamellar (smectic-A) phase in DNA solutions—are two exciting developments that have opened avenues for discovery. Here, we report on a wider investigation of the nature and temperature dependence of smectic ordering in concentrated solutions of various “gapped” DNA (GDNA) constructs. We examine symmetric GDNA constructs consisting of two 48-base pair duplex segments bridged by a single-stranded sequence of 2 to 20 thymine bases. Two distinct smectic layer structures are observed for DNA concentration in the range ∼ 230 to ∼ 280 mg/mL. One exhibits an interlayer periodicity comparable with two-duplex lengths (“bilayer” structure), and the other has a period similar to a single-duplex length (“monolayer” structure). The bilayer structure is observed for gap length &10 bases and melts into the cholesteric phase at a temperature between 30 ◦C and 35 ◦C. The monolayer structure predominates for gap length .10 bases and persists to >40 ◦C. We discuss models for the two layer structures and mechanisms for their stability. We also report results for asymmetric gapped constructs and for constructs with terminal overhangs, which further support the model layer structures. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	“gapped” DNA \| smectic liquid crystal \| phase transitions \| SAXS
语种	英语
scopus关键词	article; DNA strand; liquid crystal; periodicity; phase transition; temperature dependence
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180185
作者单位	Department of Physics, Kent State University, Kent, OH 44242, United States; Department of Physics, University of Colorado, Boulder, CO 80309, United States; Department ofChemistry and Biochemistry, Kent State University, Kent, OH 44242, United States; National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, United States; Materials Science Graduate Program, Kent State University, Kent, OH 44242, United States; Advanced Materials and Liquid Crystals Institute, Kent StateUniversity, Kent, OH 44242, United States
推荐引用方式 GB/T 7714	Gyawali P.,Saha R.,Smith G.P.,等. Mono- and bilayer smectic liquid crystal ordering in dense solutions of “gapped” DNA duplexes[J],2021,118(12).
APA	Gyawali P..,Saha R..,Smith G.P..,Salamonczyk M..,Kharel P..,...&Sprunt S..(2021).Mono- and bilayer smectic liquid crystal ordering in dense solutions of “gapped” DNA duplexes.Proceedings of the National Academy of Sciences of the United States of America,118(12).
MLA	Gyawali P.,et al."Mono- and bilayer smectic liquid crystal ordering in dense solutions of “gapped” DNA duplexes".Proceedings of the National Academy of Sciences of the United States of America 118.12(2021).