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DOI	10.1073/pnas.2026337118
	Isolation and characterization of Helicobacter suis from human stomach
	Rimbara E.; Suzuki M.; Matsui H.; Nakamura M.; Morimoto M.; Sasakawa C.; Masuda H.; Nomura S.; Osaki T.; Nagata N.; Shibayama K.; Tokunaga K.
发表日期	2021
ISSN	00278424
卷号	118 期号:13
英文摘要	Helicobacter suis, a bacterial species naturally hosted by pigs, can colonize the human stomach in the context of gastric diseases such as gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Because H. suis has been successfully isolated from pigs, but not from humans, evidence linking human H. suis infection to gastric diseases has remained incomplete. In this study, we successfully in vitro cultured H. suis directly from human stomachs. Unlike Helicobacter pylori, the viability of H. suis decreases significantly on neutral pH; therefore, we achieved this using a low-pH medium for transport of gastric biopsies. Ultimately, we isolated H. suis from three patients with gastric diseases, including gastric MALT lymphoma. Successful eradication of H. suis yielded significant improvements in endoscopic and histopathological findings. Oral infection of mice with H. suis clinical isolates elicited gastric and systemic inflammatory responses; in addition, progression of gastric mucosal metaplasia was observed 4 mo postinfection. Because H. suis could be isolated from the stomachs of infected mice, our findings satisfied Koch’s postulates. Although further prospective clinical studies are needed, H. suis, like H. pylori, is likely a gastric pathogen in humans. Furthermore, comparative genomic analysis of H. suis using complete genomes of clinical isolates revealed that the genome of each H. suis isolate contained highly plastic genomic regions encoding putative strain-specific virulence factors, including type IV secretion system–associated genes, and that H. suis isolates from humans and pigs were genetically very similar, suggesting possible pig-to-human transmission. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Gastric diseases; Helicobacter suis; Zoonosis
语种	英语
scopus关键词	adult; animal experiment; animal model; animal tissue; Article; bacterial clearance; bacterial strain; bacterial viability; bacterial virulence; bacterium culture; bacterium identification; bacterium isolation; clinical article; controlled study; disease exacerbation; endoscopy; female; gastritis; genomics; Helicobacter; Helicobacter pylori; Helicobacter suis; histopathology; human; in vitro study; inflammation; intestine flora; intestine metaplasia; male; marginal zone lymphoma; middle aged; mouse; nonhuman; pH; priority journal; stomach biopsy; type IV secretion system; zoonotic transmission
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180072
作者单位	Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, 208-0011, Japan; Antimicrobial Research Center, National Institute of Infectious Diseases, Tokyo, 189-0002, Japan; Omura Satoshi Memorial Institute, Kitasato University, Tokyo, 108-8641, Japan; School of Pharmacy, Kitasato University, Tokyo, 108-8641, Japan; Department of Research Associate Product Development, Nippon Institute for Biological Science, Tokyo, 198-0024, Japan; Medical Mycology Research Center, Chiba University, Chiba, 263-8522, Japan; Department of Gastrointestinal Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, 113-8654, Japan; Department of Gastrointestinal Surgery, Nippon Medical University, Tokyo, 113-8602, Japan; Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, 181-8611, Japan; Department of Pathology, National Institute of Infectious Diseases, Tokyo, 208-0011, Japan; Department of General Medicine, Kyorin University School of Medicine, Tokyo, 181-8611, Japan
推荐引用方式 GB/T 7714	Rimbara E.,Suzuki M.,Matsui H.,et al. Isolation and characterization of Helicobacter suis from human stomach[J],2021,118(13).
APA	Rimbara E..,Suzuki M..,Matsui H..,Nakamura M..,Morimoto M..,...&Tokunaga K..(2021).Isolation and characterization of Helicobacter suis from human stomach.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA	Rimbara E.,et al."Isolation and characterization of Helicobacter suis from human stomach".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).