气候变化领域集成服务门户(CCPortal): PPARγ marks splenic precursors of multiple nonlymphoid-tissue Treg compartments

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DOI	10.1073/pnas.2025197118
	PPARγ marks splenic precursors of multiple nonlymphoid-tissue Treg compartments
	Li C.; Muñoz-Rojas A.R.; Wang G.; Mann A.O.; Benoist C.; Mathis D.
发表日期	2021
ISSN	00278424
卷号	118 期号:13
英文摘要	Foxp3+CD4+ regulatory T cells (Tregs) regulate most types of immune response as well as several processes important for tissue homeostasis, for example, metabolism and repair. Dedicated Treg compartments—with distinct transcriptomes, T cell receptor repertoires, and growth/survival factor dependencies—have been identified in several nonlymphoid tissues. These Tregs are specifically adapted to function and operate in their home tissue—When, where, and how do they take on their specialized characteristics? We recently reported that a splenic Treg population expressing low levels of the transcription factor PPARγ (peroxisome proliferator-activated receptor gamma) contains precursors of Tregs residing in visceral adipose tissue. This finding made sense given that PPARγ, the “master regulator” of adipocyte differentiation, is required for the accumulation and function of Tregs in visceral adipose tissue but not in lymphoid tissues. Here we use single-cell RNA sequencing, single-cell Tcra and Tcrb sequencing, and adoptive-transfer experiments to show that, unexpectedly, the splenic PPARγlo Treg population is transcriptionally heterogeneous and engenders Tregs in multiple nonlymphoid tissues beyond visceral adipose tissue, such as skin and liver. The existence of a general pool of splenic precursors for nonlymphoid-tissue Tregs opens possibilities for regulating their emergence experimentally or therapeutically. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Immunoregulation; Precursor; Single-cell RNA-seq; Tissue Treg cell
语种	英语
scopus关键词	peroxisome proliferator activated receptor gamma; RNA; T lymphocyte receptor; transcription factor FOXP3; adipocyte; adoptive transfer; animal experiment; Article; CD4+ T lymphocyte; cell differentiation; controlled study; homeostasis; immune response; intra-abdominal fat; liver; lymphoid tissue; mouse; nonhuman; priority journal; regulatory T lymphocyte; RNA sequencing; skin; spleen cell
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180071
作者单位	Department of Immunology, Harvard Medical School, Boston, MA 02115, United States
推荐引用方式 GB/T 7714	Li C.,Muñoz-Rojas A.R.,Wang G.,et al. PPARγ marks splenic precursors of multiple nonlymphoid-tissue Treg compartments[J],2021,118(13).
APA	Li C.,Muñoz-Rojas A.R.,Wang G.,Mann A.O.,Benoist C.,&Mathis D..(2021).PPARγ marks splenic precursors of multiple nonlymphoid-tissue Treg compartments.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA	Li C.,et al."PPARγ marks splenic precursors of multiple nonlymphoid-tissue Treg compartments".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).