气候变化领域集成服务门户(CCPortal): TMEM70 and TMEM242 help to assemble the rotor ring of human ATP synthase and interact with assembly factors for complex I

CCPortal

DOI	10.1073/pnas.2100558118
	TMEM70 and TMEM242 help to assemble the rotor ring of human ATP synthase and interact with assembly factors for complex I
	Carroll J.; He J.; Ding S.; Fearnley I.M.; Walker J.E.
发表日期	2021
ISSN	00278424
卷号	118 期号:13
英文摘要	Human mitochondrial ATP synthase is a molecular machine with a rotary action bound in the inner organellar membranes. Turning of the rotor, driven by a proton motive force, provides energy to make ATP from ADP and phosphate. Among the 29 component proteins of 18 kinds, ATP6 and ATP8 are mitochondrial gene products, and the rest are nuclear gene products that are imported into the organelle. The ATP synthase is assembled from them via intermediate modules representing the main structural elements of the enzyme. One such module is the c8-ring, which provides the membrane sector of the enzyme’s rotor, and its assembly is influenced by another transmembrane (TMEM) protein, TMEM70. We have shown that subunit c interacts with TMEM70 and another hitherto unidentified mitochondrial transmembrane protein, TMEM242. Deletion of TMEM242, similar to deletion of TMEM70, affects but does not completely eliminate the assembly of ATP synthase, and to a lesser degree the assembly of respiratory enzyme complexes I, III, and IV. Deletion of TMEM70 and TMEM242 together prevents assembly of ATP synthase and the impact on complex I is enhanced. Removal of TMEM242, but not of TMEM70, also affects the introduction of subunits ATP6, ATP8, j, and k into the enzyme. TMEM70 and TMEM242 interact with the mitochondrial complex I assembly (the MCIA) complex that supports assembly of the membrane arm of complex I. The interactions of TMEM70 and TMEM242 with MCIA could be part of either the assembly of ATP synthase and complex I or the regulation of their levels. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Assembly; ATP synthase; Human mitochondria; TMEM242; TMEM70
语种	英语
scopus关键词	cytochrome c oxidase; membrane protein; protein TMEM242; protein TMEM70; proton transporting adenosine triphosphate synthase; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); ubiquinol cytochrome c reductase; unclassified drug; Article; controlled study; enzyme structure; human; human cell; priority journal; protein protein interaction; rotor ring
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180070
作者单位	Medical Research Council, Mitochondrial Biology Unit, University of Cambridge, Cambridge, CB2 0XY, United Kingdom
推荐引用方式 GB/T 7714	Carroll J.,He J.,Ding S.,et al. TMEM70 and TMEM242 help to assemble the rotor ring of human ATP synthase and interact with assembly factors for complex I[J],2021,118(13).
APA	Carroll J.,He J.,Ding S.,Fearnley I.M.,&Walker J.E..(2021).TMEM70 and TMEM242 help to assemble the rotor ring of human ATP synthase and interact with assembly factors for complex I.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA	Carroll J.,et al."TMEM70 and TMEM242 help to assemble the rotor ring of human ATP synthase and interact with assembly factors for complex I".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).