气候变化领域集成服务门户(CCPortal): DNA affinity purification sequencing and transcriptional profiling reveal new aspects of nitrogen regulation in a filamentous fungus

CCPortal

DOI	10.1073/pnas.2009501118
	DNA affinity purification sequencing and transcriptional profiling reveal new aspects of nitrogen regulation in a filamentous fungus
	Huberman L.B.; Wu V.W.; Kowbel D.J.; Lee J.; Daum C.; Grigoriev I.V.; O’Malley R.C.; Louise Glass N.
发表日期	2021
ISSN	00278424
卷号	118 期号:13
英文摘要	Sensing available nutrients and efficiently utilizing them is a challenge common to all organisms. The model filamentous fungus Neurospora crassa is capable of utilizing a variety of inorganic and organic nitrogen sources. Nitrogen utilization in N. crassa is regulated by a network of pathway-specific transcription factors that activate genes necessary to utilize specific nitrogen sources in combination with nitrogen catabolite repression regulatory proteins. We identified an uncharacterized pathway-specific transcription factor, amn-1, that is required for utilization of the nonpreferred nitrogen sources proline, branched-chain amino acids, and aromatic amino acids. AMN-1 also plays a role in regulating genes involved in responding to the simple sugar mannose, suggesting an integration of nitrogen and carbon metabolism. The utilization of nonpreferred nitrogen sources, which require metabolic processing before being used as a nitrogen source, is also regulated by the nitrogen catabolite regulator NIT-2. Using RNA sequencing combined with DNA affinity purification sequencing, we performed a survey of the role of NIT-2 and the pathway-specific transcription factors NIT-4 and AMN-1 in directly regulating genes involved in nitrogen utilization. Although previous studies suggested promoter binding by both a pathway-specific transcription factor and NIT-2 may be necessary for activation of nitrogen-responsive genes, our data show that pathway-specific transcription factors regulate genes involved in the catabolism of specific nitrogen sources, while NIT-2 regulates genes involved in utilization of all nonpreferred nitrogen sources, such as nitrogen transporters. Together, these transcription factors form a nutrient sensing network that allows N. crassa cells to regulate nitrogen utilization. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	DAPseq; Nitrogen utilization; Nutrient sensing; RNAseq; Transcriptional networks
语种	英语
scopus关键词	AMN 1 protein; aromatic amino acid; branched chain amino acid; mannose; NIT 2 protein; NIT 4 protein; nitrogen; proline; transcription factor; unclassified drug; amn 1 gene; Article; binding affinity; carbon metabolism; cell function; controlled study; DNA binding; DNA purification; DNA sequencing; DNA transcription; filamentous fungus; gene; gene activation; gene control; gene function; genetic association; Neurospora crassa; nitrogen metabolism; nitrogen utilization; nonhuman; priority journal; RNA sequencing; signal transduction
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180066
作者单位	Plant and Microbial Biology Department, University of California, Berkeley, CA 94720, United States; Energy Biosciences Institute, University of California, Berkeley, CA 94720, United States; US Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Huberman L.B.,Wu V.W.,Kowbel D.J.,et al. DNA affinity purification sequencing and transcriptional profiling reveal new aspects of nitrogen regulation in a filamentous fungus[J],2021,118(13).
APA	Huberman L.B..,Wu V.W..,Kowbel D.J..,Lee J..,Daum C..,...&Louise Glass N..(2021).DNA affinity purification sequencing and transcriptional profiling reveal new aspects of nitrogen regulation in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA	Huberman L.B.,et al."DNA affinity purification sequencing and transcriptional profiling reveal new aspects of nitrogen regulation in a filamentous fungus".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).