气候变化领域集成服务门户(CCPortal): Drosophila Fezf functions as a transcriptional repressor to direct layer-specific synaptic connectivity in the fly visual system

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DOI	10.1073/PNAS.2025530118
	Drosophila Fezf functions as a transcriptional repressor to direct layer-specific synaptic connectivity in the fly visual system
	Santiago I.J.; Zhang D.; Saras A.; Pontillo N.; Xu C.; Chen X.; Weirauch M.T.; Mistry M.; Ginty D.D.; Pecot M.Y.; Peng J.
发表日期	2021
ISSN	00278424
卷号	118 期号:13
英文摘要	The layered compartmentalization of synaptic connections, a common feature of nervous systems, underlies proper connectivity between neurons and enables parallel processing of neural information. However, the stepwise development of layered neuronal connections is not well understood. The medulla neuropil of the Drosophila visual system, which comprises 10 discrete layers (M1 to M10), where neural computations underlying distinct visual features are processed, serves as a model system for understanding layered synaptic connectivity. The first step in establishing layer-specific connectivity in the outer medulla (M1 to M6) is the innervation by lamina (L) neurons of one of two broad, primordial domains that will subsequently expand and transform into discrete layers. We previously found that the transcription factor dFezf cell-autonomously directs L3 lamina neurons to their proper primordial broad domain before they form synapses within the developing M3 layer. Here, we show that dFezf controls L3 broad domain selection through temporally precise transcriptional repression of the transcription factor slp1 (sloppy paired 1). In wild-type L3 neurons, slp1 is transiently expressed at a low level during broad domain selection. When dFezf is deleted, slp1 expression is up-regulated, and ablation of slp1 fully rescues the defect of broad domain selection in dFezf-null L3 neurons. Although the early, transient expression of slp1 is expendable for broad domain selection, it is surprisingly necessary for the subsequent L3 innervation of the M3 layer. DFezf thus functions as a transcriptional repressor to coordinate the temporal dynamics of a transcriptional cascade that orchestrates sequential steps of layer-specific synapse formation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	DFezf; Growth cone; Laminar organization; Neural connectivity; Transcription
语种	英语
scopus关键词	transcription factor; transcription factor slp1; unclassified drug; Article; brain nerve cell; controlled study; Drosophila; Fezf gene; gene; gene deletion; gene expression regulation; gene function; gene targeting; genetic association; genetic transcription; nonhuman; phenotype; priority journal; sequence analysis; synapse; visual system; wild type
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180047
作者单位	Department of Neurobiology, Harvard Medical School, Boston, MA 02115, United States; Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States; Division of Biomedical Informatics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States; Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45267, United States; Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115, United States; HHMI, Harvard Medical School, Boston, MA 02115, United States
推荐引用方式 GB/T 7714	Santiago I.J.,Zhang D.,Saras A.,et al. Drosophila Fezf functions as a transcriptional repressor to direct layer-specific synaptic connectivity in the fly visual system[J],2021,118(13).
APA	Santiago I.J..,Zhang D..,Saras A..,Pontillo N..,Xu C..,...&Peng J..(2021).Drosophila Fezf functions as a transcriptional repressor to direct layer-specific synaptic connectivity in the fly visual system.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA	Santiago I.J.,et al."Drosophila Fezf functions as a transcriptional repressor to direct layer-specific synaptic connectivity in the fly visual system".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).