气候变化领域集成服务门户(CCPortal): Differential regulation of local mRNA dynamics and translation following long-term potentiation and depression

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DOI	10.1073/PNAS.2017578118
	Differential regulation of local mRNA dynamics and translation following long-term potentiation and depression
	Donlin-Asp P.G.; Polisseni C.; Klimek R.; Heckel A.; Schuman E.M.
发表日期	2021
ISSN	00278424
卷号	118 期号:13
英文摘要	Decades of work have demonstrated that messenger RNAs (mRNAs) are localized and translated within neuronal dendrites and axons to provide proteins for remodeling and maintaining growth cones or synapses. It remains unknown, however, whether specific forms of plasticity differentially regulate the dynamics and translation of individual mRNA species. To address this, we targeted three individual synaptically localized mRNAs, CamkIIa, β-actin, Psd95, and used molecular beacons to track endogenous mRNA movements. We used reporters and CRISPR/Cas9 gene editing to track mRNA translation in cultured neurons. We found alterations in mRNA dynamic properties occurred during two forms of synaptic plasticity, long-term potentiation (cLTP) and depression (mGluR-LTD). Changes in mRNA dynamics following either form of plasticity resulted in an enrichment of mRNA in the vicinity of dendritic spines. Both the reporters and tagging of endogenous proteins revealed the transcript-specific stimulation of protein synthesis following cLTP or mGluR-LTD. As such, the plasticity-induced enrichment of mRNA near synapses could be uncoupled from its translational status. The enrichment of mRNA in the proximity of spines allows for localized signaling pathways to decode plasticity milieus and stimulate a specific translational profile, resulting in a customized remodeling of the synaptic proteome. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Local protein synthesis; mRNA; mRNA beacons; mRNA localization; Neuron protein synthesis
语种	英语
scopus关键词	messenger RNA; animal cell; Article; controlled study; CRISPR-CAS9 system; dendritic spine; depression; gene control; gene editing; gene location; long term potentiation; nerve cell culture; nerve cell plasticity; nonhuman; priority journal; protein synthesis; rat; RNA translation; synapse
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180041
作者单位	Department of Synaptic Plasticity, Max Planck Institute for Brain Research, Frankfurt, 60438, Germany; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Frankfurt, 60438, Germany
推荐引用方式 GB/T 7714	Donlin-Asp P.G.,Polisseni C.,Klimek R.,et al. Differential regulation of local mRNA dynamics and translation following long-term potentiation and depression[J],2021,118(13).
APA	Donlin-Asp P.G.,Polisseni C.,Klimek R.,Heckel A.,&Schuman E.M..(2021).Differential regulation of local mRNA dynamics and translation following long-term potentiation and depression.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA	Donlin-Asp P.G.,et al."Differential regulation of local mRNA dynamics and translation following long-term potentiation and depression".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).