气候变化领域集成服务门户(CCPortal): ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection

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DOI	10.1073/PNAS.2023157118
	ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection
	Mena E.L.; Donahue C.J.; Vaites L.P.; Li J.; Rona G.; O'Leary C.; Lignitto L.; Miwatani-Minter B.; Paulo J.A.; Dhabaria A.; Ueberheide B.; Gygi S.P.; Pagano M.; Harper J.W.; Davey R.A.; Elledge S.J.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	In order to understand the transmission and virulence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is necessary to understand the functions of each of the gene products encoded in the viral genome. One feature of the SARS-CoV-2 genome that is not present in related, common coronaviruses is ORF10, a putative 38-amino acid protein-coding gene. Proteomic studies found that ORF10 binds to an E3 ubiquitin ligase containing Cullin-2, Rbx1, Elongin B, Elongin C, and ZYG11B (CRL2ZYG11B). Since CRL2ZYG11B mediates protein degradation, one possible role for ORF10 is to "hijack" CRL2ZYG11B in order to target cellular, antiviral proteins for ubiquitylation and subsequent proteasomal degradation. Here, we investigated whether ORF10 hijacks CRL2ZYG11B or functions in other ways, for example, as an inhibitor or substrate of CRL2ZYG11B. While we confirm the ORF10-ZYG11B interaction and show that the N terminus of ORF10 is critical for it, we find no evidence that ORF10 is functioning to inhibit or hijack CRL2ZYG11B. Furthermore, ZYG11B and its paralog ZER1 are dispensable for SARS-CoV-2 infection in cultured cells. We conclude that the interaction between ORF10 and CRL2ZYG11B is not relevant for SARS-CoV-2 infection in vitro. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cul2; Orf10; Sars-cov-2; Zer1; Zyg11b
语种	英语
scopus关键词	cell cycle protein; CUL2 protein, human; cullin; multiprotein complex; viral protein; ZYG11A protein, human; genetics; HEK293 cell line; human; metabolism; open reading frame; Cell Cycle Proteins; COVID-19; Cullin Proteins; HEK293 Cells; Humans; Multiprotein Complexes; Open Reading Frames; SARS-CoV-2; Viral Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180032
作者单位	Department of Genetics, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, United States; Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, United States; Department of Microbiology, National Emerging Infectious Disease Laboratories, Boston University Medical Campus, Boston, MA 02115, United States; Department of Cell Biology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, United States; Department of Biochemistry and Molecular Pharmacology, New York University Grossman School of Medicine, New York, NY 10016, United States; Howard Hughes Medical Institute, New York University Grossman School of Medicine, New York, NY 10016, United States; Division of Advanced Research Technologies, Proteomics Laboratory, New York University Grossman School of Medicine, New York, NY 10016, United States; Department of Neurology, New York University Grossman School of Medicine, New York, NY 10016, United States
推荐引用方式 GB/T 7714	Mena E.L.,Donahue C.J.,Vaites L.P.,et al. ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection[J],2021,118(17).
APA	Mena E.L..,Donahue C.J..,Vaites L.P..,Li J..,Rona G..,...&Elledge S.J..(2021).ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Mena E.L.,et al."ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).